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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jun 12, 2024 |
| Title |
MR1 presents sulfated bile acid as a self antigen for MAIT cells [single-cell RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To investigate the contribution of novel antigen for MAIT cells; Cholic acid sulfate, we established Sult2a KO mice lacking antigen-producing the enzyme. We performed scRNA sequencing of whole thymocyte and liver cells from these and wild type mice. Sult2a was deficient in hepatocytes, whereas there was no change in other genes or cell proportions, suggesting that deficient of Sult2a did not affect to other genes. The analysis of thymocye implied that cholic acid sulfate may have affected the MAIT subsets.
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| Overall design |
Whole thymocyte and density fractionated-liver cells from 5-week-old wild type and Sult2aΔ1-8/Δ1-8 mice were analyzed using scRNA sequencing.
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| Contributor(s) |
Ito E, Liu Y, Motooka D, Okuzaki D, Yamasaki S |
| Citation(s) |
38277465 |
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| Submission date |
May 26, 2022 |
| Last update date |
Jun 13, 2024 |
| Contact name |
Daisuke Motooka |
| E-mail(s) |
dry_team@ngs.gen-info.osaka-u.ac.jp
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| Organization name |
NGS core facility, Research Institute for Microbial Diseases, Osaka University
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| Street address |
3-1, Yamadaoka
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| City |
Suita |
| State/province |
Osaka |
| ZIP/Postal code |
5650871 |
| Country |
Japan |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (18)
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| GSM6202264 |
Liver-4,KO-1, scRNA-Seq |
| GSM6202265 |
Liver-5,KO-2, scRNA-Seq |
| GSM6202266 |
Liver-6,WT-3, scRNA-Seq |
| GSM6202267 |
Thymus, scRNA-Seq |
| GSM6202268 |
Thymus, scRNA-Seq_HTO |
| GSM6202269 |
Liver-1,WT-1, scTCR-Seq |
| GSM6202270 |
Liver-2,WT-2, scTCR-Seq |
| GSM6202271 |
Liver-3,KO-3, scTCR-Seq |
| GSM6202272 |
Liver-4,KO-1, scTCR-Seq |
| GSM6202273 |
Liver-5,KO-2, scTCR-Seq |
| GSM6202274 |
Liver-6,WT-3, scTCR-Seq |
| GSM6202275 |
Thymus, scTCR-Seq |
| GSM7971836 |
WT_Thymus_GEX [GA41203] |
| GSM7971837 |
WT_Thymus_TCR [GA41205] |
| GSM7971838 |
WT_Thymus_HTO [GA41207] |
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| Relations |
| BioProject |
PRJNA842718 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE204917_RAW.tar |
36.0 Mb |
(http)(custom) |
TAR (of CSV, TSV, TXT) |
| GSE204917_thymus_cite-seq_condition.csv.gz |
149 b |
(ftp)(http) |
CSV |
| GSE204917_thymus_cite-seq_surface_protein.csv.gz |
242 b |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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