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| Status |
Public on Aug 29, 2022 |
| Title |
Influence of vaccination and time on gene expression of healthy backgrounded beef cattle |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Twelve clinically healthy backgrounded cattle (n=6 vaccinated, n=6 non-vaccinated), over four time points (median age in days: 107, 114, 183, 230) were selected for whole blood (Tempus Blood RNA Tubes) mRNA sequencing via NovaSeq 6000 S4 workflow. Sequenced raw reads (~35M/sample) were analyzed for the influence of time (immunological development) and vaccination with a commercial modified live viral multivalent vaccine on host gene expression.
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| |
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| Overall design |
Comparative time-course RNA-Seq analysis on lysed whole blood samples across four time points. Cattle (n=12) were evenly distributed based on vaccine administration at median day 107 and boostering at median day 183, or no vaccination.
Grant:
#2019-67015-29845
Title: "Impact of management decisions during the cow-calf, backgrounding, and feedlot phases of beef production on BRD morbidity and mortality risks"
Funding source: USDA National Institute of Food and Agriculture (NIFA) Agriculture and Food Research Initiative (AFRI).
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| |
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| Contributor(s) |
Scott MA |
| Citation(s) |
36225796 |
| Submission date |
May 27, 2022 |
| Last update date |
Oct 28, 2022 |
| Contact name |
Matthew Adam Scott |
| E-mail(s) |
matthewscott@tamu.edu
|
| Organization name |
Texas A&M University
|
| Department |
Large Animal Clinical Sciences
|
| Street address |
3201 Russell Long Blvd
|
| City |
Canyon |
| State/province |
TX |
| ZIP/Postal code |
79015 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL26012 |
Illumina NovaSeq 6000 (Bos taurus) |
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| Samples (48)
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| Relations |
| BioProject |
PRJNA843055 |
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