|
| Status |
Public on Jan 07, 2023 |
| Title |
Changes in gene expression profile caused by mechanical stress in the ligamentum flavum -long term rabbit model- |
| Organism |
Oryctolagus cuniculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To identify the changes in gene expression profile caused by mechanical stress in the ligamentum flavum, we performed resection of the L3-4 supraspinal muscle and L2-3, L4-5 posterolateral fusion with instrumentation to concentrate the mechanical stress with segmental instability at the L3-4 level. The control group underwent only surgical exposure as a sham operation. Both groups of rabbits were sacrificed at 1 year after surgery and total RNA were extracted from ligamentum flavum.
|
| |
|
| Overall design |
mRNA profiles of rabbit ligamentum flavum obtained at the intervertebral stress concentration level (ISC level) , the fused level and the control level were generated by deep sequencing using Illumina Miseq. Sequence data were mapped on the Rabbit genome (Apr. 2009 (Broad/oryCun2)) using Tophat and analyzed by Cufflinks.
|
| |
|
| Contributor(s) |
Suzuki A, Yabu A, Imai Y |
| Citation(s) |
36583686 |
| BioProject |
PRJNA839445 |
| Submission date |
May 31, 2022 |
| Last update date |
Apr 08, 2023 |
| Contact name |
Akinobu Suzuki |
| E-mail(s) |
a-suzuki@omu.ac.jp
|
| Phone |
+81-6-6645-3851
|
| Organization name |
Osaka Metropolitan University
|
| Department |
Department of Orthopaedic surgery
|
| Street address |
1-4-3 Asahimachi, Abeno-ku
|
| City |
Osaka |
| State/province |
Osaka |
| ZIP/Postal code |
545-8585 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL28443 |
Illumina MiSeq (Oryctolagus cuniculus) |
|
| Samples (9)
|
|
Less...
More...