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Series GSE20560 Query DataSets for GSE20560
Status Public on Jan 01, 2011
Title Dll1 mice cloned by nuclear transfer
Organism Mus musculus
Experiment type Expression profiling by array
Summary Production of viable and fertile mice have been reported by nuclear replacement, however, the reasons behind the frequent deformations among foetuses and progeny are not well understood. Activation and in vitro culture methods are also important. Origin of the nuclear donor cells affects success rates, and their survival in culture varies. It has been reported that offspring of nuclear replacement parental mice were normal and fertile. All epigenetic problems in the parents seem to be erased when cell nuclei go through the germ line.
cDNA microarray analysis compared expression patterns of Dll1 ko versus Dll1 ko NT (NT = nuclear transfer) and wt versus wt NT of liver and spleen. The aim of this study was analysis of possible epigenetic effects by cloning in the offspring of the 3rd generation.
If the 3rd generation cloned animals are indeed phenotypically equivalent to conventional transgenic mouse models then this would open the possibility to develop novel techniques of genetic engineering based on somatic gene targeting and nuclear replacement.
 
Overall design four male animals of each cohort (Dll1 conventional transgenic mouse, Dll1 nuclear transfer mouse, wildtype, wildtye nuclear transfer mouse). Two technical replicates including a colour flip experiment. For each analysed organ (liver, spleen) eight experiments including four biological replicates. As reference RNA pools were used
 
Contributor(s) Beckers J, Horsch M, Dinnyes A
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Submission date Mar 01, 2010
Last update date Mar 22, 2012
Contact name Martin Irmler
Organization name Helmholtz Zentrum München GmbH
Department Institute of Experimental Genetics
Lab Gene Regulation & Epigenetics
Street address Ingolstaedter Landstrasse 1
City Neuherberg
State/province Bayern
ZIP/Postal code 85764
Country Germany
 
Platforms (1)
GPL4937 GSF/IEG mouse 21K array (+RZPD)
Samples (32)
GSM512068 Dll1 cloned vs Dll1 (ref)_liver_rep1
GSM512069 Dll1 cloned vs Dll1 (ref)_liver_rep2
GSM512070 Dll1 cloned vs Dll1 (ref)_liver_rep3
Relations
BioProject PRJNA125209

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE20560_RAW.tar 57.9 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table
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