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| Status |
Public on Jun 25, 2023 |
| Title |
Inverse Agonists of the Androgen Receptor |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Other
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| Summary |
We report the efficacy of androgen receptor inverse agonists (ARIAs) in altering androgen receptor (AR) transcriptional activity. Through RNA-sequencing studies in a panel of prostate cancer cell lines and patient derived xenograft models, we demonstrate ARIAs succcessfully inhibit androgen response gene sets. ChIP-seq and CUT&RUN studies demonstrate H3K27ac decreases at AR-target genes in response to treatment. AR and NCOR1/2 localizes at these regions of acetylation loss. HiChIP further revealed loss of 3D connectivity in reponse to ARIA treatment,
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| Overall design |
RNA-seq studies of prostate cancer (PCa) cell lines or patient derived xenograft models treated with controls or androgen receptor inverse agonists (ARIA). CUT&RUN or ChIP-seq with control or ARIA treatment in PCa cell lines with IP targets including AR, H3K27ac, NCOR1, and NCOR2/SMRT. HiChIP with control or ARIA treatment in PCa cell lines with IP targets of AR, H3K27ac, and CTCF.
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| Contributor(s) |
Gryder BE, Kim H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jun 13, 2022 |
| Last update date |
Jun 29, 2023 |
| Contact name |
Hyunmin Kim |
| E-mail(s) |
human.gim@Gmail.com
|
| Organization name |
Case western reserve university
|
| Department |
genetics and genome sciences
|
| Street address |
10900 Euclid Ave.
|
| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
44106-4955 |
| Country |
USA |
| |
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| Platforms (3) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (57)
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| Relations |
| BioProject |
PRJNA848752 |
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