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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 04, 2012 |
| Title |
The Sp100 component of ND10/PML bodies is a potent tumor suppressor |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Identifying the functions of proteins, which define specific subnuclear structures and territories, is important for understanding eukaryotic nuclear dynamics. Sp100 is a prototypical protein of ND10/PML bodies and co-localizes with the proto-oncogenic protein PML and Daxx, proteins with critical roles in oncogenic transformation, interferon-mediated viral resistance and response to PML-directed cancer therapeutics. Sp100 isoforms contain PHD, Bromo and HMG domains and are highly sumoylated at ND10/PML bodies, all characteristics suggestive of a role in chromatin mediated gene regulation. However, no clear role for the Sp100 component of PML bodies in oncogenesis has been defined. Using isoform-specific knockdown techniques, we show that most human diploid fibroblasts, which lack Sp100, rapidly senesce and discuss gene expression changes associated with this rapid senescence.
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| Overall design |
microarray analysis of mRNAs expression in BJV (collected at passage 47) and BJ-S (collected at passage 35). In addition, samples were treated for 24 h with 1000 U/ml IFNB. All RNAs were collected in triplicates of biological samples.
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| Contributor(s) |
Negorev DG, Vladimirova OV, Kossenkov AV, Nikonova EV, Demarest RM, Capobianco AJ, Showe LC, Rauscher III FJ |
| Citation(s) |
21118961 |
| Submission date |
Mar 03, 2010 |
| Last update date |
Jun 19, 2013 |
| Contact name |
Louise C Showe |
| E-mail(s) |
lshowe@wistar.org
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| Phone |
215-898-3791
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| Organization name |
The Wistar Institute
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| Lab |
Dr. Louise Showe
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| Street address |
3601 Spruce St
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL6102 |
Illumina human-6 v2.0 expression beadchip |
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| Samples (12)
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| GSM517848 |
BJ-S.1 - BJ cells with Sp100 knock out, rep 1 |
| GSM517849 |
BJ-S.2 - BJ cells with Sp100 knock out, rep 2 |
| GSM517850 |
BJ-S.3 - BJ cells with Sp100 knock out, rep 3 |
| GSM517851 |
BJ-S+inf.1 - BJ cells with Sp100 knock out, treated with IFNb, rep 1 |
| GSM517852 |
BJ-S+inf.2 - BJ cells with Sp100 knock out, treated with IFNb, rep 2 |
| GSM517853 |
BJ-S+inf.3 - BJ cells with Sp100 knock out, treated with IFNb, rep 3 |
| GSM517876 |
BJV.1 - BJ cells with empty vector, rep 1 |
| GSM517877 |
BJV.2 - BJ cells with empty vector, rep 2 |
| GSM517878 |
BJV.3 - BJ cells with empty vector, rep 3 |
| GSM517879 |
BJV+inf.1 - BJ cells with empty vector, treated with IFNb, rep 1 |
| GSM517880 |
BJV+inf.2 - BJ cells with empty vector, treated with IFNb, rep 2 |
| GSM517881 |
BJV+inf.3 - BJ cells with empty vector, treated with IFNb, rep 3 |
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| Relations |
| BioProject |
PRJNA124793 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE20613_RAW.tar |
27.4 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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