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| Status |
Public on Mar 10, 2010 |
| Title |
Differential Expression upon infection with Salmonella typhimurium (t1) |
| Organism |
Dictyostelium discoideum |
| Experiment type |
Expression profiling by array
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| Summary |
Dictyostelium discoideum is a professional phagocyte and it uses phagocytosis as a way to get food. However, there are a number of bacteria that subvert this professional phagocyte. In this study we investigated whether Salmonella typhimurium is also pathogenic for D. discoideum.
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| Overall design |
AX2 cells were grown in a 1000-fold excess of E.Coli B/r in Soerensen phosphate buffer under shaking. Cell were washed and resuspended at a concentration of 2 x 10^6 per ml with a 1000-fold excess of either S. typhimurium or E.Coli B/r in Soerensen phosphate buffer. After 0, 1, 2 and 3 hours cells were washed free of bacteria and total RNA was extracted for DNA microarray analysis.
For each timepoint three samples were taken and the experiment was done twice so that we had six samples for each timepoint (e.g. S1, S2, S3 and S1', S2', S3' ).
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| Citation(s) |
21824247 |
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| Submission date |
Mar 05, 2010 |
| Last update date |
Sep 06, 2013 |
| Contact name |
Ludwig Eichinger |
| E-mail(s) |
ludwig.eichinger@uni-koeln.de
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| Phone |
+49 221 478 6928
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| Organization name |
Institute for Biochemistry 1
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| Lab |
AG Eichinger
|
| Street address |
Joseph-Stelzmann-Strasse 52
|
| City |
Cologne |
| ZIP/Postal code |
50931 |
| Country |
Germany |
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| Platforms (1) |
| GPL1972 |
Dictyostelium discoideum cDNA Microarray 6000 |
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| Samples (8)
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| GSM517971 |
SAE 13513778 GEO E1 t1 - cy3 vs S3 t1 - cy5 |
| GSM518106 |
SAE 13513872 GEO E1 t1 - cy5 vs S3 t1 - cy3 |
| GSM518107 |
SAE 13720994 GEO E2' t1 - cy3 vs S3' t1 - cy5 |
| GSM518108 |
SAE 13720997 GEO E2' t1 - cy5 vs S3' t1 - cy3 |
| GSM518109 |
SAE 13727464 GEO E2 t1 - cy5 vs S2 t1 - cy3 |
| GSM518110 |
SAE 13727665 GEO E1' t1 - cy3 vs S1' t1 - cy5 |
| GSM518111 |
SAE 13727666 GEO E1' t1 - cy5 vs S1' t1 - cy3 |
| GSM518112 |
SAE 13727669 GEO E2 t1 - cy3 vs S2 t1 - cy5 |
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| This SubSeries is part of SuperSeries: |
| GSE20688 |
Salmonella infected Dictyostelium discoideum |
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| Relations |
| BioProject |
PRJNA129603 |
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