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| Status |
Public on Mar 10, 2010 |
| Title |
Investigation of differential expression: Differentiating cells vs Salmonella infected cells (t6) |
| Organism |
Dictyostelium discoideum |
| Experiment type |
Expression profiling by array
|
| Summary |
Dictyostelium discoideum is a professional phagocyte and it uses phagocytosis as a way to get food. However, there are a number of bacteria that subvert this professional phagocyte. In this study we investigated whether Salmonella typhimurium is also pathogenic for D. discoideum.
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| Overall design |
AX2 cells were grown in a 1000-fold excess of E.Coli B/r in Soerensen phosphate buffer under shaking. Cells were washed and resuspended at a concentration of 2 x 10^6 per ml with a 1000-fold excess of S. typhimurium or in phosphate buffer. After 0, 3, 6, 9 and 12 hours, cells were washed free of bacteria and total RNA was extracted. RNA from the 3 and 6 hour time points was used for DNA microarray analysis.
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| Citation(s) |
21824247 |
| |
| Submission date |
Mar 05, 2010 |
| Last update date |
Sep 06, 2013 |
| Contact name |
Ludwig Eichinger |
| E-mail(s) |
ludwig.eichinger@uni-koeln.de
|
| Phone |
+49 221 478 6928
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| Organization name |
Institute for Biochemistry 1
|
| Lab |
AG Eichinger
|
| Street address |
Joseph-Stelzmann-Strasse 52
|
| City |
Cologne |
| ZIP/Postal code |
50931 |
| Country |
Germany |
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| Platforms (1) |
| GPL1972 |
Dictyostelium discoideum cDNA Microarray 6000 |
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| Samples (6)
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| GSM518347 |
SAE 13972509 GEO D6’ – cy3 vs S6’ – cy5 |
| GSM518348 |
SAE 13972510 GEO D6’ – cy5 vs S6’ – cy3 |
| GSM518349 |
SAE 13972543 GEO D6 – cy3 vs S6 – cy5 |
| GSM518350 |
SAE 13972544 GEO D6 – cy5 vs S6 – cy3 |
| GSM518365 |
SAE 13972551 GEO D6’ – cy3 vs S6’ – cy5 |
| GSM518366 |
SAE 13972552 GEO D6’ – cy5 vs S6’ – cy3 |
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| This SubSeries is part of SuperSeries: |
| GSE20688 |
Salmonella infected Dictyostelium discoideum |
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| Relations |
| BioProject |
PRJNA129609 |
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