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| Status |
Public on Mar 10, 2010 |
| Title |
Investigation of differential expression: Differentiating cells vs Salmonella infected cells (t3) |
| Organism |
Dictyostelium discoideum |
| Experiment type |
Expression profiling by array
|
| Summary |
Dictyostelium discoideum is a professional phagocyte and it uses phagocytosis as a way to get food. However, there are a number of bacteria that subvert this professional phagocyte. In this study we investigated whether Salmonella typhimurium is also pathogenic for D. discoideum.
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| Overall design |
AX2 cells were grown in a 1000-fold excess of E.Coli B/r in Soerensen phosphate buffer under shaking. Cells were washed and resuspended at a concentration of 2 x 10^6 per ml with a 1000-fold excess of S. typhimurium or in phosphate buffer. After 0, 3, 6, 9 and 12 hours, cells were washed free of bacteria and total RNA was extracted. RNA from the 3 and 6 hour time points was used for DNA microarray analysis.
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| Citation(s) |
21824247 |
| |
| Submission date |
Mar 05, 2010 |
| Last update date |
Sep 06, 2013 |
| Contact name |
Ludwig Eichinger |
| E-mail(s) |
ludwig.eichinger@uni-koeln.de
|
| Phone |
+49 221 478 6928
|
| Organization name |
Institute for Biochemistry 1
|
| Lab |
AG Eichinger
|
| Street address |
Joseph-Stelzmann-Strasse 52
|
| City |
Cologne |
| ZIP/Postal code |
50931 |
| Country |
Germany |
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| Platforms (1) |
| GPL1972 |
Dictyostelium discoideum cDNA Microarray 6000 |
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| Samples (7)
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| GSM518372 |
SAE 13727645 GEO D3 – cy3 vs S3 – cy5 |
| GSM518376 |
SAE 13972466 GEO D3’ – cy3 vs S3’ – cy5 |
| GSM518377 |
SAE 13972467 GEO D3’ – cy5 vs S3’ – cy3 |
| GSM518378 |
SAE 13972526 GEO D3 – cy3 vs S3 – cy5 |
| GSM518379 |
SAE 13972527 GEO D3 – cy5 vs S3 – cy3 |
| GSM518380 |
SAE 13972528 GEO D3’ – cy3 vs S3’ – cy5 |
| GSM518381 |
SAE 13972542 GEO D3’ – cy5 vs S3’ – cy3 |
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| This SubSeries is part of SuperSeries: |
| GSE20688 |
Salmonella infected Dictyostelium discoideum |
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| Relations |
| BioProject |
PRJNA129613 |
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