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| Status |
Public on Dec 01, 2023 |
| Title |
Analysis of gene expression in the Drosophila larval central nervous system |
| Organism |
Drosophila melanogaster |
| Experiment type |
Methylation profiling by high throughput sequencing
Expression profiling by high throughput sequencing
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| Summary |
Recent studies have brought contrasting results concerning 6-methyl-adenosine (6mA) as an epigenetic mark in eukaryote genomes. Here we investigated the genomic characteristics of 6mA in drosophila larval central nervous system in wild type and tet mutant contexts using PacBio SMRT-seq. In parallel, we performed RNA-seq experiments to assess gene expression in these two conditions.
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| Overall design |
Drosophila melanogaster w1118 and tet null third instar larvae were raised on standard medium and their central nervous system was dissected at the third instar larval stage. High molecular weight genomic DNA was prepared and analyzed by SMRT sequencing using PacBio sequencing platform. Total RNA was extracted and analyzed by deep sequencing using Illumina HiSeq sequencing platform.
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| Contributor(s) |
Waltzer L, Renaud Y |
| Citation(s) |
38126351 |
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| Submission date |
Jun 24, 2022 |
| Last update date |
Jan 02, 2024 |
| Contact name |
yoan renaud |
| E-mail(s) |
yoan.renaud@uca.fr
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| Organization name |
igred
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| Street address |
place henri dunand
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| City |
clermont ferrand |
| ZIP/Postal code |
63000 |
| Country |
France |
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| Platforms (2) |
| GPL17275 |
Illumina HiSeq 2500 (Drosophila melanogaster) |
| GPL28354 |
Sequel (Drosophila melanogaster) |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA852437 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE206852_TET_fusion_all.xlsx |
6.1 Mb |
(ftp)(http) |
XLSX |
| GSE206852_WT_fusion_all.xlsx |
5.5 Mb |
(ftp)(http) |
XLSX |
| GSE206852_fpkm_wt_tet.txt.gz |
543.2 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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