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| Status |
Public on Oct 07, 2022 |
| Title |
Integration of single-cell transcriptomes and biological function reveals distinct behavioral patterns in bone marrow endothelial cells [scRNA-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Heterogeneity of endothelial cell populations reflects their diverse functions in maintaining tissue’s homeostasis. However, their phenotypic, molecular, and functional properties are not entirely mapped. We used a new Tie2-CreERT2;Rosa26-tdTomato reporter mouse to trace, profile, and cultivated primary ECs from different organs. As platform paradigm, we used this strategy to study bone marrow endothelial cells (BMEC). Single-cell mRNA sequencing of primary BMEC revealed that their diversity and native molecular signatures is transitorily preserved in an ex-vivo culture that conserves key cell-to-cell microenvironment interactions. Macrophages sustained primary BMEC cellular diversity and expansion and were critical to preserve sinusoidal-like BMEC ex-vivo. Expression of endomucin discriminated BMEC in populations exhibiting mutually exclusive properties and distinct tip and stalk signatures. In contrast to arterial-like, sinusoidal-like BMECs were short-lived, formed 2D-networks, contributed to in-vivo angiogenesis and supported hematopoietic stem/progenitor cells in-vitro. This platform can be extended to other organs’ ECs to decode mechanistic information and explore therapeutics.
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| Overall design |
Single-cell mRNA sequencing of primary BMEC cultures and bulk sequencing of Emcn+ and Emcn- BMEC sorted from P0 WBM, cultured MSC and lung.
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| Contributor(s) |
Kim Y, Zara G, Branciamore S, O'Meally D, Feng Y, Kuan C, Luo Y, Nelson M, Brummer A, Rockne R, Bouman Chen Z, Zheng Y, Cardoso AA, Carlesso N |
| Citation(s) |
36433940 |
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| Submission date |
Jun 27, 2022 |
| Last update date |
Dec 15, 2022 |
| Contact name |
Denis OMeally |
| Phone |
6262188434
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| Organization name |
City of Hope
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| Department |
Arthur Riggs Diabetes & Metabolism Research Institute
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| Lab |
Diabetes & Cancer Discovery Science
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| Street address |
1500 E Duarte Rd
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| City |
Duarte |
| State/province |
California |
| ZIP/Postal code |
91024 |
| Country |
USA |
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| Platforms (2) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE206977 |
Integration of single-cell transcriptomes and biological function reveals distinct behavioral patterns in bone marrow endothelial cells |
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| Relations |
| BioProject |
PRJNA853189 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE206976_RAW.tar |
54.1 Mb |
(http)(custom) |
TAR (of H5) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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