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Status |
Public on Jul 09, 2022 |
Title |
Genetic and functional analyses of archaeal ATP-dependent RNA Ligase |
Organism |
Thermococcus kodakarensis |
Experiment type |
Non-coding RNA profiling by high throughput sequencing
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Summary |
In this study, we generated allelic knockouts of ATP-dependent RNA ligase (Rnl) in hyperthermophilic archaeon T. kodakarensis and analyze the small RNAs.
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Overall design |
T. kodakarensis gene encoding for RNA ligase TK1545 was knock-out by homologous recombination, and the RNA samples were analyzed by RNA seq. The differentially expressed circular RNAs between the two strains were analyzed.
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Contributor(s) |
Liu Y, Takagi Y, Ho K |
Citation missing |
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Submission date |
Jul 06, 2022 |
Last update date |
Aug 04, 2022 |
Contact name |
Yancheng Liu |
E-mail(s) |
liuyancheng999@gmail.com
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Organization name |
University of Tsukuba
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Street address |
1-1-1 Tennodai
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City |
Tsukuba |
ZIP/Postal code |
305-8577 |
Country |
Japan |
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Platforms (1) |
GPL32423 |
AB SOLiD 4 System (Thermococcus kodakarensis) |
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Samples (4)
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Relations |
BioProject |
PRJNA856170 |