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| Status |
Public on Feb 28, 2011 |
| Title |
Interactions between reactive oxygen species stress and brassinosteroid hormone signaling in Arabidopsis seedlings |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
We were interested in investigating the transcriptome responses to exogenous applications of brassinosteroid hormone when Arabidopsis seedlings are pre-stressed with a reactive oxygen species, hydrogen peroxide. We were interested in seeing which subsets of BR-responsive gene transcripts were most affected and how BR-responsive gene transcripts responded to increasing concentrations of hydrogen peroxide both as a whole and individually.
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| Overall design |
Liquid culture Arabidopsis seedlings are grown under standard conditions. Hydrogen peroxide is added at various concentrations to pre-stress the seedlings. Following this pretreatment, the seedlings are then treated with brassinosteroid (BR) hormone (epi-brassinolide, BL). Following this treatment, seedlings are harvested and total RNA is extracted for genome-wide transcriptome analysis.
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| Contributor(s) |
Larue CT, Huber SC |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Mar 11, 2010 |
| Last update date |
Aug 15, 2018 |
| Contact name |
Steven C. Huber |
| E-mail(s) |
schuber1@life.uiuc.edu, clarue@illinois.edu
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| Organization name |
USDA-ARS
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| Lab |
Steve Huber Lab
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| Street address |
1201 W. Gregory Drive, 197 ERML
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| City |
Urbana |
| State/province |
IL |
| ZIP/Postal code |
61801 |
| Country |
USA |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (16)
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| GSM520751 |
1 H2O2, 0 BL, replicate 1 |
| GSM520752 |
5 H2O2, 0 BL, replicate 1 |
| GSM520753 |
25 H2O2, 0 BL, replicate 1 |
| GSM520754 |
50 H2O2, 0 BL, replicate 1 |
| GSM520755 |
100 H2O2, 0 BL, replicate 1 |
| GSM520756 |
0 H2O2, 1 BL, replicate 1 |
| GSM520757 |
0 H2O2, 1 BL, replicate 2 |
| GSM520758 |
0 H2O2, 1 BL, replicate 3 |
| GSM520759 |
1 H2O2, 1 BL, replicate 1 |
| GSM520760 |
5 H2O2, 1 BL, replicate 1 |
| GSM520761 |
25 H2O2, 1 BL, replicate 1 |
| GSM520762 |
50 H2O2, 1 BL, replicate 1 |
| GSM520763 |
100 H2O2, 1 BL, replicate 1 |
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| Relations |
| Affiliated with |
GSE69995 |
| BioProject |
PRJNA125043 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE20756_RAW.tar |
30.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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