|
| Status |
Public on Aug 31, 2022 |
| Title |
Dose-dependent consequences of sub-chronic fentanyl exposure on neuron and glial co-cultures |
| Organism |
Rattus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
It remains poorly understood what and how changes in brain activity from chronic fentanyl use influences the respective behavioral outcome. We examined the functional and molecular changes to cortical neural network activity following sub-chronic exposure to two fentanyl concentrations, a low (0.01 µM) and high (10 µM) dose from primary rat co-cultures, containing cortical neurons, astrocytes, and oligodendrocyte-lineage cell.
|
| |
|
| Overall design |
Primary rat co-cultures, containing cortical neurons, astrocytes, and oligodendrocyte precursor cells, at 24-26 days in vitro were treated with a low (0.01 µM) or high (10 µM) dose for four days before RNA was extracted.
|
| |
|
| Contributor(s) |
Enright H, Lam D, Sebastian A |
| Citation(s) |
36032789 |
| |
| Submission date |
Jul 06, 2022 |
| Last update date |
Aug 31, 2022 |
| Contact name |
Stephen Patrick Wilson |
| E-mail(s) |
wilson265@llnl.gov
|
| Organization name |
LLNL
|
| Department |
BBTD
|
| Street address |
7000 East Ave
|
| City |
Livermore |
| State/province |
CA |
| ZIP/Postal code |
94550 |
| Country |
USA |
| |
|
| Platforms (1) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA856310 |
Less...
More...