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| Status |
Public on Nov 29, 2022 |
| Title |
Matrix from Urine Stem Cells Boosts Tissue-Specific Stem Cell Mediated Functional Cartilage Reconstruction |
| Organism |
Oryctolagus cuniculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Articular cartilage has a limited capacity to self-heal once damaged. Tissue-specific stem cells are a solution for cartilage regeneration; however, ex vivo expansion resulting in cell senescence remains a challenge as a large quantity of high-quality tissue-specific stem cells are needed for cartilage regeneration. Our previous report demonstrated that decellularized extracellular matrix (dECM) deposited by human synovium-derived stem cells (SDSCs), adipose-derived stem cells (ADSCs), urine-derived stem cells (UDSCs), or dermal fibroblasts (DFs) provided a solution to rejuvenate human SDSCs in proliferation and chondrogenic potential. This study focused on the evaluation of ex vivo rejuvenation of rabbit infrapatellar fat pad-derived stem cells (IPFSCs) by the abovementioned dECMs to be used in functional cartilage repair in a rabbit osteochondral defect model and the potential cellular and molecular mechanisms underlying this rejuvenation. We found that dECM rejuvenation promoted rabbit IPFSCs’ cartilage engineering and functional regeneration in both in vitro and in vivo models, particularly for the dECM deposited by UDSCs, which was further confirmed by proteomics data. RNASeq analysis indicated that both mesenchymal-epithelial transition (MET) and inflammation-mediated macrophage activation and polarization are potentially involved in the dECM-mediated promotion of IPFSCs’ chondrogenic capacity, which needs further investigation.
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| Overall design |
Rabbit IPFSCs grown on four varied dECMs deposited by human ASCs, DFs, SDSCs, and USCs or tissue culture plastic (PL) were incubated in a pellet culture system with chondrogenic induction medium for 21 days. Both cell and pellet samples were used for RNASeq analyses.
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| Contributor(s) |
Pei M, Pei YA, Zhou S, Mikaeliagah E, Erickson C, Giertych B, Akhter H, Wang L, Stewart A, Parenti J, Wang B, Wen S, Sim S, Quenneville E, Hansen KC, Hu G |
| Citation(s) |
36474659 |
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| Submission date |
Jul 08, 2022 |
| Last update date |
Mar 01, 2023 |
| Contact name |
Gangqing Hu |
| E-mail(s) |
michael.hu@hsc.wvu.edu
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| Organization name |
West Virginia University
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| Department |
MicroBiology, Immunology, and Cell Biology
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| Lab |
2072A, HSC North, Floor 2
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| Street address |
64 Medical Center Drive
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| City |
Morgantown |
| State/province |
West Virginia |
| ZIP/Postal code |
26506-9177 |
| Country |
USA |
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| Platforms (1) |
| GPL21255 |
Illumina HiSeq 2500 (Oryctolagus cuniculus) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA857092 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE207804_RNAseq_all_rpkm.txt.gz |
1.5 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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