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| Status |
Public on Mar 12, 2010 |
| Title |
H3K9me3 (new lot).Clone_8 ChIP-chip |
| Project |
modENCODE
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| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
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| Summary |
modENCODE_submission_940
This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648
Project Goal: We aim to determe the locations of 125 chromosomal proteins and histone modifications across the Drosophila melanogaster genome. The proteins and modifications under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages.
For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf
Keywords: CHIP-chip
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| Overall design |
EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Cell Line: CME-W1-Cl.8+; Tissue: dorsal mesothoracic disc; Developmental Stage: third instar larval stage; Sex: Male; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Cell Line CME-W1-Cl.8+; Antibody H3K9me3 (new lot)
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| Web link |
http://www.modencode.org
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| Contributor(s) |
Karpen G, Elgin S, Gortchakov A, Shanower G, Tolstorukov M, Kharchenko P, Kuroda M, Pirrotta V, Park P, Minoda A, Riddle N, Schwartz Y, Alekseyenko A, Kennedy C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| BioProject |
PRJNA63467 |
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| Submission date |
Mar 11, 2010 |
| Last update date |
Jul 08, 2015 |
| Contact name |
DCC modENCODE |
| E-mail(s) |
help@modencode.org
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| Phone |
416-673-8579
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| Organization name |
Ontario Institute for Cancer Research
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| Lab |
modENCODE DCC
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| Street address |
MaRS Centre, South Tower, 101 College Street, Suite 800
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| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 0A3 |
| Country |
Canada |
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| Platforms (1) |
| GPL6629 |
[DM_tiling2_MR] Affymetrix Drosophila Tiling 2.0R Array |
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| Samples (4)
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| GSM521035 |
Cell Line CME-W1-Cl.8+;Antibody H3K9me3 (new lot) extraction1_array1 |
| GSM521036 |
Cell Line CME-W1-Cl.8+;Antibody H3K9me3 (new lot) extraction2_array1 |
| GSM521037 |
Cell Line CME-W1-Cl.8+;Antibody H3K9me3 (new lot) extraction3_array1 |
| GSM521038 |
Cell Line CME-W1-Cl.8+;Antibody H3K9me3 (new lot) extraction4_array1 |
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| Supplementary file |
Size |
Download |
File type/resource |
| GSE20822_RAW.tar |
194.9 Mb |
(http)(custom) |
TAR (of BEDGRAPH, CEL) |
| GSE20822_repset.8061134.smoothedM.bedgraph.gz |
41.5 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE20822_repset.8061134.smoothedM.clusters.gff3.gz |
88.2 Kb |
(ftp)(http) |
GFF3 |
| Processed data provided as supplementary file |
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