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| Status |
Public on May 18, 2023 |
| Title |
Stuxnet fine-tunes Notch dose during development using a functional Polycomb response element |
| Organism |
Drosophila melanogaster |
| Experiment type |
Other
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| Summary |
Evolutionarily conserved Notch signaling is highly sensitive to changes
in Notch receptor dose caused by intrinsic and environmental
fluctuations. It is well known that epigenetic regulation responds
dynamically to genetic, cellular and environmental stresses. However,
it is unclear whether the Notch receptor dose is directly regulated at the
epigenetic level. Here, by studying the role of the upstream epigenetic
regulator Stuxnet (Stx) in Drosophila developmental signaling, we find
that Stx promotes Notch receptor mRNA expression by counteracting
the activity of Polycomb repressive complex 1 (PRC1). In addition, we
provide evidence that Notch is a direct PRC1 target by identifying and
validating in vivo the only bona fide Polycomb response element (PRE)
among the seven Polycomb group (PcG)-binding sites revealed by
DamID-seq and ChIP-seq analysis. Importantly, in situ deletion of this
PRE results in increased Notch expression and phenotypes resembling
Notch hyperactivation in cell fate specification. These results not only
underscore the importance of epigenetic regulation in fine-tuning the
Notch activity dose, but also the need to assess the physiological
significance of omics-based PcG binding in development.
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| Overall design |
Three samples: Dam, Dam-Pc, and Dam-Pc and Stx drove by nub-Gal4.
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| Web link |
http://10.1242/dev.201297
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| Contributor(s) |
He T, Yi M, Liu M, Zhu AJ |
| Citation(s) |
37260148 |
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| Submission date |
Jul 18, 2022 |
| Last update date |
Aug 17, 2023 |
| Contact name |
Tao He |
| E-mail(s) |
2006391234@pku.edu.cn
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| Organization name |
Peking university
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| Street address |
Summer Palace Road No. 5
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| City |
Beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
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| Platforms (1) |
| GPL17275 |
Illumina HiSeq 2500 (Drosophila melanogaster) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA859671 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE208357_RAW.tar |
9.1 Mb |
(http)(custom) |
TAR (of BEDGRAPH) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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