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| Status |
Public on Mar 12, 2010 |
| Title |
H3K4me2.ab.S2 ChIP-chip |
| Project |
modENCODE
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| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
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| Summary |
modENCODE_submission_965
This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648
Project Goal: We aim to determe the locations of 125 chromosomal proteins and histone modifications across the Drosophila melanogaster genome. The proteins and modifications under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages.
For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf
Keywords: CHIP-chip
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| Overall design |
EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Cell Line: S2-DRSC; Tissue: embryo-derived cell-line; Developmental Stage: late embryonic stage; Sex: Male; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Cell Line S2-DRSC; Antibody H3K4me2
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| Web link |
http://www.modencode.org
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| Contributor(s) |
Karpen G, Elgin S, Gortchakov A, Shanower G, Tolstorukov M, Kharchenko P, Kuroda M, Pirrotta V, Park P, Minoda A, Riddle N, Schwartz Y, Alekseyenko A, Kennedy C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| BioProject |
PRJNA63467 |
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| Submission date |
Mar 11, 2010 |
| Last update date |
Jul 08, 2015 |
| Contact name |
DCC modENCODE |
| E-mail(s) |
help@modencode.org
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| Phone |
416-673-8579
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| Organization name |
Ontario Institute for Cancer Research
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| Lab |
modENCODE DCC
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| Street address |
MaRS Centre, South Tower, 101 College Street, Suite 800
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| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 0A3 |
| Country |
Canada |
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| Platforms (1) |
| GPL6629 |
[DM_tiling2_MR] Affymetrix Drosophila Tiling 2.0R Array |
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| Samples (4)
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| GSM521100 |
Cell Line S2-DRSC;Antibody H3K4me2 extraction1_array1 |
| GSM521101 |
Cell Line S2-DRSC;Antibody H3K4me2 extraction2_array1 |
| GSM521102 |
Cell Line S2-DRSC;Antibody H3K4me2 extraction3_array1 |
| GSM521103 |
Cell Line S2-DRSC;Antibody H3K4me2 extraction4_array1 |
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| Supplementary file |
Size |
Download |
File type/resource |
| GSE20838_RAW.tar |
188.9 Mb |
(http)(custom) |
TAR (of BEDGRAPH, CEL) |
| GSE20838_repset.7766223.smoothedM.bedgraph.gz |
41.4 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE20838_repset.7766223.smoothedM.clusters.gff3.gz |
268.7 Kb |
(ftp)(http) |
GFF3 |
| Processed data provided as supplementary file |
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