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Status |
Public on Feb 26, 2024 |
Title |
RNA-seq for blood Ly6Chi and Ly6Clo monocyte in WT and RBP-Jfl/fl Lyz2-Cre mice |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
To evaluate the consequences of RBP-J loss of function in blood monocyte subsets, we sequenced RNA of blood Ly6Chi monocyte and Ly6Clo monocyte from WT and RBP-Jfl/fl Lyz2-Cre mice.
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Overall design |
Blood Ly6Chi and Ly6Clo monocytes were isolated from WT and RBP-Jfl/fl Lyz2-Cre mice, and total RNA was extracted using total RNA purification kit. RNA was converted into RNA-seq quantification libraries, and RNA-seq libraries were sequenced with the pair-end option.
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Contributor(s) |
Kang L, Zhang B, Kou T |
Citation(s) |
38407952 |
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Submission date |
Jul 21, 2022 |
Last update date |
Mar 20, 2024 |
Contact name |
Tiantian Kou |
Organization name |
Tsinghua university
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Street address |
Haidian District
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City |
Beijing |
ZIP/Postal code |
100084 |
Country |
China |
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Platforms (1) |
GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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Samples (4)
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GSM6369153 |
RBP-Jfl/fl Lyz2-Cre blood Ly6Chi monocyte |
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Relations |
BioProject |
PRJNA860977 |