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| Status |
Public on Jul 30, 2022 |
| Title |
Transformed cells after senescence give rise to more severe tumor phenotypes than transformed non-senescent cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Oncogenic stress-induced senescence initially inhibits tumor initiation by blocking proliferation. If these cells are not eliminated they may resume proliferation upon loss-of-tumor suppressors, and be at risk of transformation. During tumor formation, depending on the sequence of events of gain-of-oncogenes and/or loss-of-tumor suppressors, cancer cells may emerge from senescent cells. The goal of this study is to determine if transformed cells after senescence (TS) display more aggressive tumorigenic features, with a greater capacity to migrate and a higher resistance to anti-tumoral drugs than cells having undergone transformation without senescence. Here, we modeled cell transformation using mouse embryonic fibroblasts (MEFs) subjected to an inverse sequence of events: i) gain-of-RasV12 oncogene and loss-of-p53 tumor suppressor, leading to transformed cells after senescence (TS), or ii) the opposite, resulting in transformed (T) non-senescent cells.
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| Overall design |
This study is based on transciptome analysis of Mouse Embrionic Fibroblasts (MEFs) expressing 4 different combination of viral vectors: Oncogenic form of RAS (RASV12); short hairpin RNA targeting mouse p53 mRNA (shp53); first shp53 plus RASV12 (T cells); or first RASV12 plus shp53 (TS cells) .For each condition three independent biological replicates were analysed.
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| Contributor(s) |
Palazzo A, Bernard D, Flaman J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jul 29, 2022 |
| Last update date |
Aug 01, 2022 |
| Contact name |
jean-michel flaman |
| E-mail(s) |
jean-michel.flaman@lyon.unicancer.fr
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| Phone |
+33687477812
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| Organization name |
Inserm 1052
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| Department |
CRCL
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| Street address |
28 rue Laennec
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| City |
Lyon |
| ZIP/Postal code |
69373 |
| Country |
France |
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| Platforms (1) |
| GPL11202 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA863600 |
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