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Series GSE210060 Query DataSets for GSE210060
Status Public on Jul 30, 2022
Title Transformed cells after senescence give rise to more severe tumor phenotypes than transformed non-senescent cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary Oncogenic stress-induced senescence initially inhibits tumor initiation by blocking proliferation. If these cells are not eliminated they may resume proliferation upon loss-of-tumor suppressors, and be at risk of transformation. During tumor formation, depending on the sequence of events of gain-of-oncogenes and/or loss-of-tumor suppressors, cancer cells may emerge from senescent cells. The goal of this study is to determine if transformed cells after senescence (TS) display more aggressive tumorigenic features, with a greater capacity to migrate and a higher resistance to anti-tumoral drugs than cells having undergone transformation without senescence. Here, we modeled cell transformation using mouse embryonic fibroblasts (MEFs) subjected to an inverse sequence of events: i) gain-of-RasV12 oncogene and loss-of-p53 tumor suppressor, leading to transformed cells after senescence (TS), or ii) the opposite, resulting in transformed (T) non-senescent cells.
Overall design This study is based on transciptome analysis of Mouse Embrionic Fibroblasts (MEFs) expressing 4 different combination of viral vectors: Oncogenic form of RAS (RASV12); short hairpin RNA targeting mouse p53 mRNA (shp53); first shp53 plus RASV12 (T cells); or first RASV12 plus shp53 (TS cells) .For each condition three independent biological replicates were analysed.
Contributor(s) Palazzo A, Bernard D, Flaman J
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Submission date Jul 29, 2022
Last update date Aug 01, 2022
Contact name jean-michel flaman
Phone +33687477812
Organization name Inserm 1052
Department CRCL
Street address 28 rue Laennec
City Lyon
ZIP/Postal code 69373
Country France
Platforms (1)
GPL11202 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
Samples (12)
GSM6415983 MEF-shp53_1
GSM6415984 MEF-shp53_2
GSM6415985 MEF-shp53_3
BioProject PRJNA863600

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE210060_RAW.tar 26.1 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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