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| Status |
Public on Dec 06, 2023 |
| Title |
AMPKα2 binds to chromatin to regulate gene expression in ALL cells [ChIP-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
To identify genome-wide genes regulated by direct association of AMPKα2 to chromatin, ChIP-Seq assays were performed using HA (HA-AMPKα2) and RPB1 (RNA Pol II) antibodies in CCRF-CEM/HA-AMPKα2 (CN2) cells treated with glucose deprivation or no treatment controls. ChIP-seq analysis identified around 600 genes to which loci AMPKα2 was directly bound; many of these genes with HA-AMPKα2 recruitment overlapped with genes exhibiting RNA Pol II enrichment.
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| Overall design |
ChIP-seq (using HA and RNA pol II antibody) in CCRF-CEM/HA-AMPKa2 cells treated with vehicle or Glucose deprivation condition.
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| Contributor(s) |
Sun G, Leclerc GJ, Barredo JC |
| Citation(s) |
37682252 |
| |
| Submission date |
Aug 01, 2022 |
| Last update date |
Dec 07, 2023 |
| Contact name |
Julio C Barredo |
| Organization name |
University of Miami
|
| Street address |
1580 NW 10th ave room
|
| City |
MIAMI |
| State/province |
Florida |
| ZIP/Postal code |
33136 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (5)
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| This SubSeries is part of SuperSeries: |
| GSE210214 |
AMPK directly regulate a subset of genes expression in ALL cells |
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| Relations |
| BioProject |
PRJNA864778 |
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