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Series GSE210213 Query DataSets for GSE210213
Status Public on Dec 06, 2023
Title AMPKα2 binds to chromatin to regulate gene expression in ALL cells [ChIP-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary To identify genome-wide genes regulated by direct association of AMPKα2 to chromatin, ChIP-Seq assays were performed using HA (HA-AMPKα2) and RPB1 (RNA Pol II) antibodies in CCRF-CEM/HA-AMPKα2 (CN2) cells treated with glucose deprivation or no treatment controls. ChIP-seq analysis identified around 600 genes to which loci AMPKα2 was directly bound; many of these genes with HA-AMPKα2 recruitment overlapped with genes exhibiting RNA Pol II enrichment.
 
Overall design ChIP-seq (using HA and RNA pol II antibody) in CCRF-CEM/HA-AMPKa2 cells treated with vehicle or Glucose deprivation condition.
 
Contributor(s) Sun G, Leclerc GJ, Barredo JC
Citation(s) 37682252
Submission date Aug 01, 2022
Last update date Dec 07, 2023
Contact name Julio C Barredo
Organization name University of Miami
Street address 1580 NW 10th ave room
City MIAMI
State/province Florida
ZIP/Postal code 33136
Country USA
 
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (5)
GSM6423646 CTRL_input
GSM6423647 CTRL_HA_CHIP
GSM6423648 CTRL_Pol_II_CHIP
This SubSeries is part of SuperSeries:
GSE210214 AMPK directly regulate a subset of genes expression in ALL cells
Relations
BioProject PRJNA864778

Download family Format
SOFT formatted family file(s) SOFTHelp
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Supplementary file Size Download File type/resource
GSE210213_RAW.tar 1.9 Gb (http)(custom) TAR (of BIGWIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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