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Series GSE2103 Query DataSets for GSE2103
Status Public on Dec 24, 2004
Title yeast hsf1-R206S/F256S mutant
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by array
Summary Effect of hsf1-R206S/F256S mutation on heat-induced transcription of genes is analyzed. Total RNA was isolated from HSF1 wild type and hsf1-R206S/F256S cells grown at 39oC for 15 min. Probe cDNA was primed by oligo(dT) and was synthesized in the presence of 33P-dCTP. Hybridization was carried out using the same GF100 GeneFilter. Value higher than 30 in HSF1 wild type cells is confident. Values from duplicate experiments were averaged, and the average fold changes were calculated.
Keywords = yeast
Keywords = HSF1
Keywords: other
 
 
Contributor(s) Sakurai H
Citation(s) 15647283
Submission date Dec 22, 2004
Last update date Mar 16, 2012
Contact name Hiroshi Sakurai
E-mail(s) sakurai@kenroku.kanazawa-u.ac.jp
Phone 81-76-265-2588
Fax 81-76-234-4360
Organization name Kanazawa University
Department School of Health Sciences
Street address 5-11-80 Kodatsuno
City Kanazawa
State/province Ishikawa
ZIP/Postal code 920-0942
Country Japan
 
Platforms (1)
GPL205 GF100 GeneFilters
Samples (4)
GSM37975 HSF1 wt replicate 1
GSM37978 HSF1 wt replicate 2
GSM37979 hsf1-R206S/F256S replicate 1
Relations
BioProject PRJNA91799

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Supplementary data files not provided

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