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| Status |
Public on Jun 01, 2010 |
| Title |
DNA microarrays of three shRNA control iPS clones (Ctrl 2,3,4) and shECAD iPS clones (shECAD 4,8,9) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
E-cadherin upregulation is an early event of reprogramming of fibroblasts to induce pluripotent stem cells (iPS). Knocking down of E-cadherin by shRNA impairs iPS generation, though some colonies with great morphorlogical difference to shRNA control colonies remain. To illustrate the molecular and functional difference between shECAD iPS clones and shRNA control iPS clones, three respective iPS clones (shECAD 4,8,9 and Ctrl 2,3,4) were derived and DNA microarrays were run to analyze the transcriptional profile of these clones.
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| Overall design |
OG2 MEFs were infected with Sox2, Klf4, Oct4 and c-Myc (SKOM) plus either Luciferase shRNA (shLUC) or E-cadherin shRNA (shECAD) retrovirus. At Day 6 post infection cells were split onto feeder cells. Several colonies from SKOM+shLuc and SKOM+shECAD were picked out at Day 14 post infection respectively and three cell lines were established, namely Ctrl 2,3,4 for SKOM+shLuc iPS and shECAD 4,8,9 for SKOM+shECAD iPS. All clones were maintained on feeder cells in mESC medium. RNA were extracted from these six cell lines and DNA microarrays were run to analyze the transcriptional profile.
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| Contributor(s) |
Li R, He W |
| Citation(s) |
20621050 |
| Submission date |
Mar 25, 2010 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Duanqing Pei |
| E-mail(s) |
duanqing.pei@gmail.com
|
| Phone |
086-20-32290520
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| Fax |
086-20-32290606
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| Organization name |
Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences(GIBH.CAS)
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| Department |
Center for Molecular Medicine
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| Lab |
Lingwen Zeng's Lab
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| Street address |
Room 301, International Business Incubator, Guangzhou science park
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| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510663 |
| Country |
China |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE21067 |
Molecular mechanisms underlying reprogramming of mouse fibroblasts into pluripotent stem cells |
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| Relations |
| BioProject |
PRJNA129681 |
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