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GEO help: Mouse over screen elements for information. |
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| Status |
Public on May 23, 2023 |
| Title |
RNA-seq analysis of differentially expressed genes between WT and shTRIM21 HCT116 cells. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To investigate the biological function TRIM21 in the colorectal cancer, we established HCT116 cell lines in which the TRIM21 gene has been knocked down by shRNA.
We used a lentiviral shRNA technique to knockdown TRIM21 expression in HCT116 cells. A scrambled shRNA (shRNA/Control) was used as a control. We then performed RNA-Seq with Cloud-seq Biotech Inc. (Shanghai, China) to analyze gene expression changes in HCT116 cells. For each sample, three independent biological replicates were performed.
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| Overall design |
Comparative gene expression profiling analysis of RNA-seq data for HCT116 cells and its KD derivatives (shTRIM21).
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| Contributor(s) |
Wu H, Yang X |
| Citation(s) |
37354905 |
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| Submission date |
Aug 15, 2022 |
| Last update date |
Aug 08, 2023 |
| Contact name |
Xiaoqin Yang |
| E-mail(s) |
yangxiaoqin@suda.edu.cn
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| Organization name |
Suzhou Medical College of Soochow University
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| Street address |
No. 199, Ren'ai Road
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| City |
Suzhou |
| State/province |
Jiangsu |
| ZIP/Postal code |
215123 |
| Country |
China |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA869704 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE211267_RAW.tar |
670.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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