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| Status |
Public on Jan 11, 2023 |
| Title |
Gene expression in 832/13 INS1 beta-cells transduced with lentiviral vector conferring expression of GFP or Pax5 |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
We found PAX5 to be overexpressed in human pancreatic islets of donors from donors with type 2 diabetes compared to islets from non-diabetic individuals. Functional follow-up showed that Pax5 overexpression in rat clonal beta-cells (832/13 INS1) results in impaired insulin secretion. Microarrays were used to investigate if overexpression of Pax5 alters the gene expression profile of 832/13 INS1 beta-cells.
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| Overall design |
16 samples, 8 from cells transduced with a control (GFP) virus and 8 transduced with a Pax5 virus. Cells were transdcued with a MOI of 1.25 and samples were collected 72h post transduction
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| Contributor(s) |
Bacos K, Perfilyev A, Karagiannopoulos A, Cowan E, Ofori JK, Bertonnier-Brouty L, Rönn T, Lindqvist A, Luan C, Ruhrmann S, Ngara M, Nilsson Å, Gheibi S, Lyons CL, Lagerstedt JO, Barghouth M, Esguerra JS, Volkov P, Fex M, Mulder H, Wierup N, Krus U, Artner I, Eliasson L, Prasad R, Cataldo LR, Ling C |
| Citation(s) |
36656641 |
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| Submission date |
Aug 15, 2022 |
| Last update date |
Apr 12, 2023 |
| Contact name |
Charlotte Ling |
| E-mail(s) |
charlotte.ling@med.lu.se
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| Organization name |
Lund Univesity
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| Street address |
Jan Waldenstroms gata 35
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| City |
Malmö |
| ZIP/Postal code |
21428 |
| Country |
Sweden |
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| Platforms (1) |
| GPL23040 |
[Clariom_S_Rat] Affymetrix Clariom S Assay, Rat (Includes Pico Assay) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA869746 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE211310_RAW.tar |
18.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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