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| Status |
Public on Aug 17, 2022 |
| Title |
Mucosal immune and stress responses of Neoparamoeba perurans-infected Atlantic salmon (Salmo salar) treated with peracetic acid shed light on the host-parasite-oxidant interactions |
| Organism |
Salmo salar |
| Experiment type |
Expression profiling by array
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| Summary |
Treatment development for parasitic infestation is often limited to disease resolution as an endpoint response, and physiological and immunological consequences are not thoroughly considered. The impact of exposing Atlantic salmon affected with amoebic gill disease (AGD) to peracetic acid (PAA), an oxidative chemotherapeutic was studied. Transcriptome profiling in the gills showed significant changes triggered by AGD and PAA treatments, and the effects of PAA were more notable 24 h after treatment. Genes related to immune pathways of B- and T- cells and protein synthesis and metabolism were downregulated, where the magnitude was more remarkable in 10ppm-15mins group. Even though treatment did not fully resolve the pathologies associated with AGD, 5ppm-30mins group showed lower parasite load at 4 weeks post-treatment. Mucous cell parameters (i.e., size and density) increased within 24 h post-treatment and were significantly higher at termination, especially in AGD-affected fish, with some treatment effects influenced by the dose of PAA. Infection and treatments resulted in oxidative stress – in the early phase in the gill mucosa, while systemic reactive oxygen species (ROS) dysregulation was evident at the later stage. Infected fish responded to elevated circulating ROS by increasing antioxidant production. Exposing the fish to a crowding stress revealed interference in the post-stress responses. Lower cortisol response was displayed by AGD-affected groups. Collectively, the study established that PAA, within the evaluated treatment protocols, could not provide a convincing treatment resolution and thus require further optimisation.
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| Overall design |
AGD-affected fish were treated with PAA either by exposing them to 5 ppm for 30 mins or 10 ppm for 15 mins. Unexposed fish from both infected and uninfected groups were also included. Samples were collected at 24 h, 2 weeks and 4 weeks post treatment.
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| Contributor(s) |
Lazado C, Krasnov A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Aug 16, 2022 |
| Last update date |
Aug 20, 2022 |
| Contact name |
Aleksei Krasnov |
| E-mail(s) |
aleksei.krasnov@nofima.no
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| Phone |
+47 97602165
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| Organization name |
Nofima AS
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| Department |
Fish Health
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| Street address |
Osloveien 1
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| City |
Aas |
| ZIP/Postal code |
1430 |
| Country |
Norway |
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| Platforms (1) |
| GPL30031 |
Agilent-030860 Nofima Atlantic salmon microarray, version 6 |
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| Samples (72)
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| GSM6467166 |
Uninfected untreated, week 2. fish 6 |
| GSM6467167 |
Uninfected untreated, week 2. fish 7 |
| GSM6467168 |
Uninfected untreated, week 2. fish 8 |
| GSM6467169 |
Infected untreated, week 2, fish 31 |
| GSM6467170 |
Infected untreated, week 2, fish 32 |
| GSM6467171 |
Infected untreated, week 2, fish 33 |
| GSM6467172 |
Infected untreated, week 2, fish 36 |
| GSM6467173 |
Infected untreated, week 2, fish 37 |
| GSM6467174 |
Infected untreated, week 2, fish 38 |
| GSM6467175 |
Infected, treated with 5 ppm for 30 mins, week 2, fish 41 |
| GSM6467176 |
Infected, treated with 5 ppm for 30 mins, week 2, fish 42 |
| GSM6467177 |
Infected, treated with 5 ppm for 30 mins, week 2, fish 43 |
| GSM6467178 |
Infected, treated with 5 ppm for 30 mins, week 2, fish 46 |
| GSM6467179 |
Infected, treated with 5 ppm for 30 mins, week 2, fish 47 |
| GSM6467180 |
Infected, treated with 5 ppm for 30 mins, week 2, fish 48 |
| GSM6467181 |
Infected, treated with 10 ppm for 15 mins, week 2, fish 51 |
| GSM6467182 |
Infected, treated with 10 ppm for 15 mins, week 2, fish 52 |
| GSM6467183 |
Infected, treated with 10 ppm for 15 mins, week 2, fish 53 |
| GSM6467184 |
Infected, treated with 10 ppm for 15 mins, week 2, fish 56 |
| GSM6467185 |
Infected, treated with 10 ppm for 15 mins, week 2, fish 57 |
| GSM6467186 |
Infected, treated with 10 ppm for 15 mins, week 2, fish 58 |
| GSM6467187 |
Uninfected untreated, week 4, fish 1 |
| GSM6467188 |
Uninfected untreated, week 4, fish 2 |
| GSM6467189 |
Uninfected untreated, week 4, fish 3 |
| GSM6467190 |
Uninfected untreated, week 4, fish 6 |
| GSM6467191 |
Uninfected untreated, week 4, fish 7 |
| GSM6467192 |
Uninfected untreated, week 4, fish 8 |
| GSM6467193 |
Infected untreated, week 4, fish 31 |
| GSM6467194 |
Infected untreated, week 4, fish 32 |
| GSM6467195 |
Infected untreated, week 4, fish 33 |
| GSM6467196 |
Infected untreated, week 4, fish 36 |
| GSM6467197 |
Infected untreated, week 4, fish 37 |
| GSM6467198 |
Infected untreated, week 4, fish 38 |
| GSM6467199 |
Infected, treated with 5 ppm for 30 mins, week 4, fish 41 |
| GSM6467200 |
Infected, treated with 5 ppm for 30 mins, week 4, fish 42 |
| GSM6467201 |
Infected, treated with 5 ppm for 30 mins, week 4, fish 43 |
| GSM6467202 |
Infected, treated with 5 ppm for 30 mins, week 4, fish 46 |
| GSM6467203 |
Infected, treated with 5 ppm for 30 mins, week 4, fish 47 |
| GSM6467204 |
Infected, treated with 5 ppm for 30 mins, week 4, fish 48 |
| GSM6467205 |
Infected, treated with 10 ppm for 15 mins, week 4, fish 51 |
| GSM6467206 |
Infected, treated with 10 ppm for 15 mins, week 4, fish 52 |
| GSM6467207 |
Infected, treated with 10 ppm for 15 mins, week 4, fish 53 |
| GSM6467208 |
Infected, treated with 10 ppm for 15 mins, week 4, fish 56 |
| GSM6467209 |
Infected, treated with 10 ppm for 15 mins, week 4, fish 57 |
| GSM6467210 |
Infected, treated with 10 ppm for 15 mins, week 4, fish 58 |
| GSM6467211 |
Uninfected untreated, week 6, fish 1 |
| GSM6467212 |
Uninfected untreated, week 6, fish 2 |
| GSM6467213 |
Uninfected untreated, week 6, fish 3 |
| GSM6467214 |
Uninfected untreated, week 6, fish 6 |
| GSM6467215 |
Uninfected untreated, week 6, fish 7 |
| GSM6467216 |
Uninfected untreated, week 6, fish 8 |
| GSM6467217 |
Infected untreated, week 6, fish 31 |
| GSM6467218 |
Infected untreated, week 6, fish 32 |
| GSM6467219 |
Infected untreated, week 6, fish 33 |
| GSM6467220 |
Infected untreated, week 6, fish 36 |
| GSM6467221 |
Infected untreated, week 6, fish 37 |
| GSM6467222 |
Infected untreated, week 6, fish 38 |
| GSM6467223 |
Infected, treated with 5 ppm for 30 mins, week 6, fish 41 |
| GSM6467224 |
Infected, treated with 5 ppm for 30 mins, week 6, fish 42 |
| GSM6467225 |
Infected, treated with 5 ppm for 30 mins, week 6, fish 43 |
| GSM6467226 |
Infected, treated with 5 ppm for 30 mins, week 6, fish 46 |
| GSM6467227 |
Infected, treated with 5 ppm for 30 mins, week 6, fish 47 |
| GSM6467228 |
Infected, treated with 5 ppm for 30 mins, week 6, fish 48 |
| GSM6467229 |
Infected, treated with 10 ppm for 15 mins, week 6, fish 51 |
| GSM6467230 |
Infected, treated with 10 ppm for 15 mins, week 6, fish 52 |
| GSM6467231 |
Infected, treated with 10 ppm for 15 mins, week 6, fish 53 |
| GSM6467232 |
Infected, treated with 10 ppm for 15 mins, week 6, fish 56 |
| GSM6467233 |
Infected, treated with 10 ppm for 15 mins, week 6, fish 57 |
| GSM6467234 |
Infected, treated with 10 ppm for 15 mins, week 6, fish 58 |
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| Relations |
| BioProject |
PRJNA869938 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE211350_Lowess_normalized_data.xlsx |
5.6 Mb |
(ftp)(http) |
XLSX |
| GSE211350_RAW.tar |
51.8 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data are available on Series record |
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