|
| Status |
Public on Aug 26, 2022 |
| Title |
Integrative analyses of transcriptome and proteome provides new insights into energy metabolism and light utilzation in cigar tobacco during different light intensities |
| Organism |
Nicotiana tabacum |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this study, we have used the comparative transcriptomic and proteomic approaches to decipher the changes in genes and protein abundances in cigar tobacco leaves under LL. In this study, DEGs and DEPs related to glycolysis, starch and sucrose metabolism, tyrosine metabolism, photosynthesis-antenna proteins, and photosynthesis pathways are significantly enriched. This study offers novel insights into both transcriptome and proteome levels response mechanisms under different light intensities.
|
| |
|
| Overall design |
The cigar tobacco H382 variety was used as test material in this study。A total of nine RNA samples were prepared from three treatments and three biological replicates (3╳3 = 9). Total RNA was extracted from the leaves samples using TRIzol Reagent (Invitrogen, Carlsbad, CA, USA) consulting manufacturer instructions. GOseq R package was used to identify significantly enriched GO terms and KOBAS software was used to test the statistical enrichment of differential expression genes in KEGG pathways. qRT-PCR was performed using SYBR Premix Ex Taq II (Takara, Japan).
|
| |
|
| Contributor(s) |
Wu X, Ma X |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Aug 23, 2022 |
| Last update date |
Aug 26, 2022 |
| Contact name |
ying xiao wu |
| E-mail(s) |
wuxiaoying2021@yeah.net
|
| Organization name |
Tobacco Research Institute, Chinese Academy of Agricultural Sciences
|
| Department |
Cultivation and nutrition center
|
| Lab |
Key Laboratory of Tobacco Biology and Processing, Ministry of Agriculture
|
| Street address |
China and South Korea the street
|
| City |
qingdao |
| State/province |
shangdong |
| ZIP/Postal code |
266000 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL29390 |
Illumina NovaSeq 6000 (Nicotiana tabacum) |
|
| Samples (9)
|
| GSM6505614 |
Cigar tobacco plants under 50 μmol m-2 s-1 light intensity [T50_1] |
| GSM6505615 |
Cigar tobacco plants under 50 μmol m-2 s-1 light intensity [T50_2] |
| GSM6505616 |
Cigar tobacco plants under 50 μmol m-2 s-1 light intensity [T50_3] |
| GSM6505617 |
Cigar tobacco plants under 100 μmol m-2 s-1 light intensity [T100_1] |
| GSM6505618 |
Cigar tobacco plants under 100 μmol m-2 s-1 light intensity [T100_2] |
| GSM6505619 |
Cigar tobacco plants under 100 μmol m-2 s-1 light intensity [T100_3] |
| GSM6505620 |
Cigar tobacco plants under 200 μmol m-2 s-1 light intensity [T200_1] |
| GSM6505621 |
Cigar tobacco plants under 200 μmol m-2 s-1 light intensity [T200_2] |
| GSM6505622 |
Cigar tobacco plants under 200 μmol m-2 s-1 light intensity [T200_3] |
|
| Relations |
| BioProject |
PRJNA872821 |
Less...
More...