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| Status |
Public on Oct 01, 2022 |
| Title |
Identify and characterize the polyploidization process of primary human proximal tubular cells (hPTC) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We generated single-cell RNA sequencing (scRNAseq) datasets of primary cultures of human proximal tubular cells at different passages which we have found to contain a polyploid fraction. Within hPTC clusters, we found genes previously associated with different phases of endoreplication-mediated polyploidy and established that YAP1 controls the polyploidization process of hPTC. These observations were verified by treating hPTC with verteporfin (a YAP1 inhibitor), YAP1 silencing and by quantifying the expression of YAP1 downstream targets in sorted polyploid hPTC
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| Overall design |
Analyze the polyploidization process of primary human proximal tubular cells (hPTC)
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| Contributor(s) |
De Chiara L, Semeraro R, Lazzeri E, Romagnani P |
| Citation(s) |
36195583, 37642236 |
| |
| Submission date |
Aug 29, 2022 |
| Last update date |
Dec 01, 2023 |
| Contact name |
Roberto Semeraro |
| E-mail(s) |
robe.semeraro@gmail.com
|
| Organization name |
Università degli studi di Firenze
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| Department |
Medicina Sperimentale e Clinica
|
| Lab |
206, secondo piano, Cubo 3
|
| Street address |
Viale Pieraccini, 6
|
| City |
Firenze |
| ZIP/Postal code |
50139 |
| Country |
Italy |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (3) |
| GSM6523053 |
human renal proximal tubule epithelial cells p6 |
| GSM6523054 |
human renal proximal tubule epithelial cells p7 |
| GSM6523055 |
human renal proximal tubule epithelial cells p8 |
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| Relations |
| BioProject |
PRJNA874743 |
