|
| Status |
Public on Feb 26, 2024 |
| Title |
Effect of SRSF1 depletion on FANCD2 mRNA and isoform expression and effect of FANCD2 depletion or expression of SRSF1 mutants on splicing |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
To investigate the cooperative function of FANCD2/SRSF1 complex in the regulation of R-loops, we performed gene expression, isoform and splicing analysis in Hela cells depleted of SRSF1 or expressing SRSF1 mutants and FA-D2 mutant (FA-D2) and wild type (FA-D2+FANCD2) cells.
|
| |
|
| Overall design |
FANCD2 gene expression and isoform analysis of RNA-seq data for HeLa cells depleted for SRSF1. Differential splicing analysis of RNA-seq data for HeLa cells expressing SRSF1 mutants or FA-D2 mutant (FA-D2) and wild type (FA-D2+FANCD2) cells.
|
| |
|
| Contributor(s) |
Olazabal A, Pillai M, Kupfer G |
| Citation(s) |
38165804 |
| |
| Submission date |
Aug 31, 2022 |
| Last update date |
Feb 26, 2024 |
| Contact name |
MANOJ PILLAI |
| E-mail(s) |
MANOJ.PILLAI@YALE.EDU
|
| Organization name |
YALE UNIVERSITY
|
| Lab |
PILLAI LAB
|
| Street address |
300 GEORGE STREET #786
|
| City |
NEW HAVEN |
| State/province |
CT |
| ZIP/Postal code |
06511 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA875477 |
Less...
More...