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| Status |
Public on Sep 04, 2022 |
| Title |
Cell types and molecular architecture of the Octopus bimaculoides visual system |
| Organism |
Octopus bimaculoides |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Cephalopods have a remarkable visual system, with a camera-type eye, high acuity vision, and a wide range of sophisticated visual behaviors. However, the cephalopod brain is organized dramatically differently from that of vertebrates, as well as other invertebrates, and little is known regarding the cell types and molecular determinants of their visual system organization beyond neuroanatomical descriptions. Here we present a comprehensive single-cell molecular atlas of the octopus optic lobe, which is the primary visual processing structure in the cephalopod brain. We combined single-cell RNA sequencing with RNA fluorescence in situ hybridization to both identify putative molecular cell types and determine their anatomical and spatial organization within the optic lobe. Our results reveal six major neuronal cell classes identified by neurotransmitter/neuropeptide usage, in addition to non-neuronal and immature neuronal populations. Moreover, we find that additional markers divide these neuronal classes into subtypes with distinct anatomical localizations, revealing cell type diversity and a detailed laminar organization within the optic lobe. We also delineate the immature neurons within this continuously growing tissue into subtypes defined by evolutionarily conserved fate specification genes as well as novel cephalopod- and octopus- specific genes. Together, these findings outline the organizational logic of the octopus visual system, based on functional determinants, laminar identity, and developmental markers/pathways. The resulting atlas presented here delineates the “parts list” of the neural circuits used for vision in octopus, providing a platform for investigations into the development and function of the octopus visual system as well as the evolution of visual processing.
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| Overall design |
Characterization of cell types in the Octopus bimaculoides visual system through both gene expression profiling analysis of single-cell RNA sequencing data and RNA fluorescent in situ hybridization
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| Contributor(s) |
Songco-Casey JO, Coffing GC, Piscopo DM, Pungor JR, Kern AD, Miller AC, Niell CM |
| Citation(s) |
36318923 |
| BioProject |
PRJNA854179 |
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| Submission date |
Sep 01, 2022 |
| Last update date |
Jan 13, 2023 |
| Contact name |
Cristopher Niell |
| E-mail(s) |
cniell@uoregon.edu
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| Organization name |
University of Oregon
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| Street address |
1565 E 13th st
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| City |
Eugene |
| State/province |
Oregon |
| ZIP/Postal code |
97403 |
| Country |
USA |
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| Platforms (2) |
| GPL25425 |
Illumina NextSeq 500 (Octopus bimaculoides) |
| GPL32623 |
Illumina HiSeq 4000 (Octopus bimaculoides) |
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| Samples (4)
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| Supplementary file |
Size |
Download |
File type/resource |
| GSE212528_1_barcodes.tsv.gz |
20.0 Mb |
(ftp)(http) |
TSV |
| GSE212528_1_features.tsv.gz |
119.5 Kb |
(ftp)(http) |
TSV |
| GSE212528_1_matrix.mtx.gz |
217.5 Mb |
(ftp)(http) |
MTX |
| GSE212528_2_barcodes.tsv.gz |
20.0 Mb |
(ftp)(http) |
TSV |
| GSE212528_2_features.tsv.gz |
119.5 Kb |
(ftp)(http) |
TSV |
| GSE212528_2_matrix.mtx.gz |
142.8 Mb |
(ftp)(http) |
MTX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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