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| Status |
Public on Oct 15, 2010 |
| Title |
Expression analysis of Thermus thermophilus HB8 wild-type strain in response to high growth temperature |
| Organism |
Thermus thermophilus HB8 |
| Experiment type |
Expression profiling by array
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| Summary |
In order to observe the heat-shock response of extremely thermohilic bacterium T. thermophilus HB8 strain, we analyzed the altered expression profile of the total mRNA in T. thermophilus HB8 wild-type strain after the growth temperature was shifted from 70°C to 80°C for 30 min.
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| Overall design |
Three wild-type strain of T. thermophilus HB8 were pre-cultured at 70°C for 16 hours in 3 mL of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (0.5 mL) were inoculated into 250 mL of TT medium and then cultivated at 70°C for 6 hours. After the cells were collected from 50 ml culture medium, the remaining medium was cultured at 80°C for 30 min. The cells were collected, and then crude RNA was extracted from each lot of cells. The expression of each mRNA at each time point was analyzed on a GeneChip.
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| Contributor(s) |
Agari Y, Ohmori M, Kuramitsu S, Shinkai A |
| Citation(s) |
21054499 |
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| Submission date |
Apr 11, 2010 |
| Last update date |
Mar 22, 2012 |
| Contact name |
Akeo Shinkai |
| E-mail(s) |
ashinkai@spring8.or.jp, y_agari@spring8.or.jp
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| URL |
http://www.srg.harima.riken.jp/
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| Organization name |
RIKEN Harima Institute
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| Department |
SPring-8 Center
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| Street address |
1-1-1, Kouto, Sayo
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| City |
Hyogo |
| ZIP/Postal code |
679-5148 |
| Country |
Japan |
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| Platforms (1) |
| GPL9209 |
[TTHB8401a520105F] Riken Thermus thermophilus HB8 4K mRNA custom array, library Rev.2 |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE21875 |
SuperSeries for the study of expression pattern analysis of Thermus thermophilus HB8 |
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| Relations |
| BioProject |
PRJNA129441 |
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