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Series GSE213026 Query DataSets for GSE213026
Status Public on Oct 05, 2023
Title Synaptic-like transmission between neural axons and arterial smooth muscle cells drives cerebral neurovascular coupling
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Normally functioning brains depend on neurovascular coupling (NVC) and its dysfunction underlies many neuropathologies. Although cell-type specificity has been implicated in NVC, how active neural information is specifically conveyed to the targeted arterioles in the brain remains poorly understood. Here, using two-photon focal optogenetics in the mouse cerebral cortex, we demonstrated that single glutamatergic axons dilate their innervating arterioles via synaptic-like transmission between neural-arteriolar smooth muscle cells junction (NsMJ). The presynaptic parental-daughter bouton makes dual innervations on postsynaptic dendrites and on arteriolar smooth muscles cells (aSMCs), which expressed many types of neuromediator receptors, including glutamate NMDA receptor subunit 1 (Grin1). Disruption of NsMJ transmission, by aSMC specific knockout of GluN1, diminished the optogenetic and whisker stimulation-caused functional hyperemia. Notably, the absence of GluN1 subunit in aSMCs protected the brain atrophy from cerebral ischemia by preventing Ca2+ overload in aSMCs during arteriolar constriction caused by the ischemia-induced spreading depolarization. Our findings reveal that NsMJ transmission drives neurovascular coupling and also open up a new avenue for studying stroke.
Overall design mRNA profiles of arteriolar smooth muscle cell, cortical cell and astrocyte
Web link
Contributor(s) Zhang D, Ge W, Jia J
Citation(s) 38168932
Submission date Sep 09, 2022
Last update date Jan 16, 2024
Contact name Dong-Dong Zhang
Phone 08613051063166
Organization name Fudan University
Street address 130 Dong'an Road
City Shanghai
State/province Shanghai
ZIP/Postal code 200032
Country China
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (17)
GSM6568824 arteriolar smooth muscle cell, sorting, rep7
GSM6568825 arteriolar smooth muscle cell, sorting, rep8
GSM6568826 cortex, homogenate, rep1
BioProject PRJNA878800

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE213026_Raw_gene_counts_matrix.txt.gz 438.1 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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