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| Status |
Public on Oct 05, 2023 |
| Title |
Synaptic-like transmission between neural axons and arterial smooth muscle cells drives cerebral neurovascular coupling |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Normally functioning brains depend on neurovascular coupling (NVC) and its dysfunction underlies many neuropathologies. Although cell-type specificity has been implicated in NVC, how active neural information is specifically conveyed to the targeted arterioles in the brain remains poorly understood. Here, using two-photon focal optogenetics in the mouse cerebral cortex, we demonstrated that single glutamatergic axons dilate their innervating arterioles via synaptic-like transmission between neural-arteriolar smooth muscle cells junction (NsMJ). The presynaptic parental-daughter bouton makes dual innervations on postsynaptic dendrites and on arteriolar smooth muscles cells (aSMCs), which expressed many types of neuromediator receptors, including glutamate NMDA receptor subunit 1 (Grin1). Disruption of NsMJ transmission, by aSMC specific knockout of GluN1, diminished the optogenetic and whisker stimulation-caused functional hyperemia. Notably, the absence of GluN1 subunit in aSMCs protected the brain atrophy from cerebral ischemia by preventing Ca2+ overload in aSMCs during arteriolar constriction caused by the ischemia-induced spreading depolarization. Our findings reveal that NsMJ transmission drives neurovascular coupling and also open up a new avenue for studying stroke.
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| Overall design |
mRNA profiles of arteriolar smooth muscle cell, cortical cell and astrocyte
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| Web link |
https://www.nature.com/articles/s41593-023-01515-0
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| Contributor(s) |
Zhang D, Ge W, Jia J |
| Citation(s) |
38168932 |
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| Submission date |
Sep 09, 2022 |
| Last update date |
Jan 16, 2024 |
| Contact name |
Dong-Dong Zhang |
| E-mail(s) |
2008010556zdd@gmail.com
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| Phone |
08613051063166
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| Organization name |
Fudan University
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| Street address |
130 Dong'an Road
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| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (17)
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| GSM6568827 |
cortex, homogenate, rep2 |
| GSM6568828 |
cortex, homogenate, rep3 |
| GSM6568829 |
cortex, homogenate, rep4 |
| GSM6568830 |
cortex, homogenate, rep5 |
| GSM6568831 |
cortex, homogenate, rep6 |
| GSM6568832 |
astrocyte, sorting, rep1 |
| GSM6568833 |
astrocyte, sorting, rep2 |
| GSM6568834 |
astrocyte, sorting, rep3 |
| GSM7493520 |
arteriolar smooth muscle cell, sorting, rep1 |
| GSM7493521 |
arteriolar smooth muscle cell, sorting, rep2 |
| GSM7493522 |
arteriolar smooth muscle cell, sorting, rep3 |
| GSM7493523 |
arteriolar smooth muscle cell, sorting, rep4 |
| GSM7493524 |
arteriolar smooth muscle cell, sorting, rep5 |
| GSM7493525 |
arteriolar smooth muscle cell, sorting, rep6 |
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| Relations |
| BioProject |
PRJNA878800 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE213026_Raw_gene_counts_matrix.txt.gz |
438.1 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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