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Series GSE213202 Query DataSets for GSE213202
Status Public on Nov 03, 2022
Title mTORC1 signaling facilitates differential stem cell differentiation to shape the developing murine lung and is associated with mitochondrial capacity
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Formation of branched organs requires sequential differentiation of stem cells. In this work, we find that the conducting airways derived from SOX2+ progenitors in the murine lungs fail to form without mTOR complex 1 (mTORC1) signaling and are replaced by lung cysts. Proximal-distal patterning through transitioning of distal SOX9+ progenitors to the proximal SOX2+ cells is disrupted. Mitochondria number and ATP production are reduced. Compromised mitochondrial capacity results in a similar defect as that in mTORC1-deficient lungs. This suggests that mTORC1 promotes differentiation of SOX9+ progenitors to form the conducting airways by modulating mitochondrial capacity. Surprisingly, in all mutants saccules are produced from lung cysts at the proper developmental time despite defective branching. SOX9+ progenitors also differentiate into alveolar epithelial type I and type II cells within saccules. These findings highlight selective utilization of energy and regulatory programs during stem cell differentiation to produce distinct structures of the mammalian lungs.
 
Overall design Examine the difference of gene expression among Rptor-, Tfam-, Cox10-deficient and their littermate control lungs
 
Contributor(s) Zhang K, Yao E, Chuang E, Chen B, Chuang EY, Chuang P
Citation(s) 36433959
Submission date Sep 12, 2022
Last update date Dec 15, 2022
Contact name Pao-Tien Chuang
Organization name University of California, San Francisco
Street address 555 Mission Bay Blvd. South
City San Francisco
ZIP/Postal code CA 94158-3118
Country USA
 
Platforms (1)
GPL23479 BGISEQ-500 (Mus musculus)
Samples (18)
GSM6574343 Rptor_control lung_rep 1
GSM6574344 Rptor_control lung_rep 2
GSM6574345 Rptor_control lung_rep 3
Relations
BioProject PRJNA879749

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE213202_Cox10_ShhCre_14.5dpc.csv.gz 872.2 Kb (ftp)(http) CSV
GSE213202_Rptor_ShhCre_14.5dpc.csv.gz 891.1 Kb (ftp)(http) CSV
GSE213202_Tfam_ShhCre_14.5dpc.csv.gz 883.4 Kb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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