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| Status |
Public on Mar 06, 2024 |
| Title |
Basal cell extrusion primes pluripotent cells for differentiation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
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| Summary |
The blueprint of the mammalian body plan is laid out during gastrulation, when a trilaminar embryo is formed. This process entails a burst of embryonic cell proliferation, the ingression of pluripotent epithelial cells at the primitive streak, and their priming towards primitive streak fates. How these different events are coordinated remains unknown. Here, we developed a 3D culture of self-renewing pluripotent and epithelial cells that recapitulates the transcriptional and architectural features of the gastrulating mouse embryo. Using this system in combination with microfabrication and in vivo experiments, we found that proliferation triggers basal extrusion in cells that express high levels of the apical polarity protein aPKC. Upon extrusion, cells are more sensitive to Bmp, Wnt, and Nodal signaling, and upregulate the expression of primitive streak markers. This mechanistic coupling between cell position and gene expression ensures that the right cell types become specified at the right place during embryonic development.
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| Overall design |
scRNA-Seq, RNA-Seq, BS-Seq
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| Contributor(s) |
Satoh N, Rosa VS, Makhlouf A, Kretzmer H, Kumar AS, Grosswendt S, Mattei A, Courbot O, Wolf S, McDole K, Langevin F, Wiacek M, Karpinski D, Elosegui-Artola A, Meissner A, Zernicka-Goetz M, Shahbazi M |
| Citation missing |
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| Submission date |
Sep 14, 2022 |
| Last update date |
Mar 07, 2024 |
| Contact name |
Helene Kretzmer |
| E-mail(s) |
kretzmer@molgen.mpg.de
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| Organization name |
Max Planck Institute for Molecular Genetics
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| Department |
Genome Regulation
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| Lab |
Meissner Lab
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| Street address |
Ihnestraße 63
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| City |
Berlin |
| ZIP/Postal code |
14195 |
| Country |
Germany |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (35)
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| Relations |
| BioProject |
PRJNA880552 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE213336_RAW.tar |
679.5 Mb |
(http)(custom) |
TAR (of BW, TAR) |
| GSE213336_RNA_TPM.tsv.gz |
3.9 Mb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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