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| Status |
Public on Jul 18, 2023 |
| Title |
Single cell sequencing of WT and hb-egfaKO zebrafish spinal cord, at 1 week after sham or transection injury. |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We identified hb-egfa as a secreted factor necessary and sufficient for spinal cord regeneration in zebrafish. To elucidate the Hb-egfa mechanism of action, we perforemd single cell sequencing of WT and hb-egfaKO zebrafish spinal cord.
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| Overall design |
Adult zebrafish were subjected to spinal cord transection. 2 mm rostral and caudal to the transection site were collected at 1 week post injury. Spinal cord of sham-injured animals was collected as control. Samples were and subjected to the 10X Genomics pipeline for single cell sequencing (4 samples in total, WT 1 wpi, WT sham, hb-egfaKO 1 wpi, hb-egfaKO sham).
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| Contributor(s) |
Cigliola V, Shoffner A, Lee N, Ou J, Becker C, Hoque J, Gonzalez T, Han Y, Sheng G, Faw T, Abd-El-Barr M, Varghese S, Asokan A, Poss KD |
| Citation(s) |
37567873 |
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| Submission date |
Sep 15, 2022 |
| Last update date |
Oct 17, 2023 |
| Contact name |
jianhong ou |
| E-mail(s) |
jianhong.ou@duke.edu
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| Phone |
5084102796
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| Organization name |
Duke University
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| Department |
Cell Biology
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| Lab |
Regeneromics
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| Street address |
465 Nanaline Duke Building, Duke University Medical Center
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| City |
Durham |
| State/province |
North Carolina |
| ZIP/Postal code |
27710 |
| Country |
USA |
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| Platforms (1) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA880627 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE213435_RAW.tar |
28.7 Mb |
(http)(custom) |
TAR (of H5) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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