|
| Status |
Public on Oct 19, 2023 |
| Title |
Low input lipidomics analysis reveals remodeling of lipid metabolism and saturation during mouse and human preimplantation embryo development |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Scd1 is responsible for forming a carbon-carbon double bound at the 9-10th position from the C-terminus of saturated fatty acids such as palmitic acid and stearic acid (C16:0 and C18:0), to generate the products of palmitoleic acid (C16:1) and oleic acid (C18:1).Here, we found SCD1 is required for in vitro blastocyst embryo development, and one of the mechanisms is through regulating unsaturated fatty acid-mediated membrane fluidity and formation of apical domain. Overall, our study provides invaluable resources for lipid reprogramming in mammalian preimplantation embryo development and mechanistic insights on regulation of embryogenesis by lipid unsaturation.
|
| |
|
| Overall design |
Examination of gene expression in wide-type and scd1 knocking down embryo
|
| |
|
| Contributor(s) |
Zhang L, Zhao J, Zhang J, Yu H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Sep 20, 2022 |
| Last update date |
Oct 19, 2023 |
| Contact name |
Hua Yu |
| E-mail(s) |
yuhua@westlake.edu.cn
|
| Phone |
18758008246
|
| Organization name |
Westlake University
|
| Street address |
No.18 Shilongshan Road
|
| City |
Hangzhou |
| ZIP/Postal code |
310024 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA882438 |
Less...
More...