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| Status |
Public on Apr 20, 2010 |
| Title |
Expression Data from WT and Sh2d1a-/- in vivo follicular helper CD4 T cells (TFH) versus non follicular helper CD4 T cells (non-TFH) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
CD4 T cell help is critical for both the generation and maintenance of germinal centers, and T follicular helper (TFH) cells are the CD4 T cell subset required for this process. SAP (SH2D1A) expression in CD4 T cells is essential for germinal center development. However, SAP-deficient mice have only a moderate defect in TFH differentiation as defined by common TFH surface markers. CXCR5+ TFH cells are found within the germinal center as well as along the boundary regions of T/B cell zones. Here we show that germinal center associated T cells (GC TFH) can be identified by their co-expression of CXCR5 and the GL7 epitope, allowing for phenotypic and functional analysis of TFH and GC TFH populations. Here we show GC TFH are a functionally discrete subset of further polarized TFH cells, with enhanced B cell help capacity and a specialized ability to produce IL-4 in a TH2-independent manner. Strikingly, SAP-deficient mice have an absence of the GC TFH subset and SAP- TFH are defective in IL-4 and IL-21 production. We further demonstrate that SLAM (Slamf1, CD150), a surface receptor that utilizes SAP signaling, is specifically required for IL-4 production by GC TFH. GC TFH cells require IL-4 and IL-21 production for optimal help to B cells. These data illustrate complexities of SAP-dependent SLAM family receptor signaling, revealing a prominent role for SLAM receptor ligation in IL-4 production by germinal center CD4 T cells but not in TFH and GC TFH differentiation.
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| Overall design |
Analysis of in vivo antigen-specific (LCMV-specific, SMARTA TCR transgenic) WT and Sh2d1a-/- follicular helper CD4 T cells (CXCR5high),versus non-follicular helper CD4 T cells (CXCR5low), eight days after viral infection.
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| Contributor(s) |
Crotty S |
| Citation(s) |
20525889 |
| Submission date |
Apr 19, 2010 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Shane Crotty |
| E-mail(s) |
shane@liai.org
|
| Organization name |
La Jolla Institute for Allergy and Immunology
|
| Department |
Vaccine Discovery
|
| Lab |
VD-Crotty
|
| Street address |
9420 Athena Circle
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE21381 |
Germinal center T follicular helper cell IL-4 production is dependent on SLAM receptor (CD150) |
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| Relations |
| BioProject |
PRJNA129503 |
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