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Series GSE213812 Query DataSets for GSE213812
Status Public on Jun 07, 2023
Title Prenatal exposure to benzo[a]pyrene depletes ovarian reserve and masculinizes embryonic ovarian germ cell transcriptome transgenerationally [RNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary People are exposed to polycyclic aromatic hydrocarbons, like benzo[a]pyrene (BaP), via inhalation of particulate matter air pollution and ingestion of grilled and smoked foods. Prenatal exposure to BaP destroys germ cells in ovaries, causing earlier onset of ovarian senescence post-natally. Developing testes are affected at higher doses than ovaries. However, it is not known if adverse effects are transmitted to subsequent generations. We orally dosed pregnant female mice (F0) with 0.033, 0.2, or 2 mg/kg-day BaP or vehicle from embryonic day (E) 6.5-11.5 (F1 offspring) or E6.5-15.5 (F2 and F3). Ovarian germ cell and follicle numbers were significantly decreased in F3 females at all doses of BaP; testicular germ cells were not affected. E13.5 germ cell RNA-sequencing revealed significantly increased expression of male-specific genes in female germ cells across generations and BaP doses. Our study demonstrated that F0 BaP exposure partially disrupted sexual identity of female germ cells transgenerationally.
Overall design Comparative gene expression profiling analysis of RNA-seq data for FACS-enriched mouse primordial germ cells with or without gestational exposure to banzo[a]pyrene.
Contributor(s) Shioda T, Lim J, Luderer U
Citation(s) 37248279
Submission date Sep 20, 2022
Last update date Jun 07, 2023
Contact name Toshi Shioda
Phone (617) 726-3425
Organization name MGH Cancer Center
Department Center for Cancer Research
Lab Molecular Profiling Lab
Street address Building 149 - 7th Floor, 13th Street
City Charlestown
State/province MA
ZIP/Postal code 02129
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (101)
GSM6594660 F1.high.C4219.1_M
GSM6594661 F1.high.C4219.2_M
GSM6594662 F1.high.C4219.4_F
BioProject PRJNA882474

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Supplementary file Size Download File type/resource
GSE213812_normalized_RNA_counts.txt.gz 7.4 Mb (ftp)(http) TXT
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