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| Status |
Public on Oct 31, 2022 |
| Title |
De novo emergence, existence, and demise of a protein-coding gene in murids [chinese hamster] |
| Organism |
Cricetulus griseus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Genes, principal units of genetic information, vary in complexity and evolutionary history. Less-complex genes (e.g. long non-coding RNA (lncRNA) expressing genes), readily emerge de novo from non-genic sequences and have high evolutionary turnover. Genesis of a gene is facilitated by adoption of functional genic sequences from retrotransposon insertions. However, protein-coding sequences in extant genomes rarely lack any connection to an ancestral protein-coding sequence. Here, we describe remarkable evolution of the murine gene D6Ertd527e and its orthologs in the rodent Muroidea superfamily. The D6Ertd527e emerged in a common ancestor of mice and hamsters as an lncRNA-expressing gene. A major contributing factor was a long terminal repeat (LTR) retrotransposon insertion carrying an oocyte-specific promoter and one of the first exons of the gene. The gene survived as an oocyte-specific lncRNA in several extant rodents while in some others the gene or its expression was lost. In the ancestral lineage of Mus musculus, the gene acquired protein-coding capacity where the bulk of the coding sequence formed through CAG (AGC) trinucleotide repeat expansion and duplications. These events gave rise to a cytoplasmic serine-rich maternal protein, which has no discernable role. Knock-out of D6Ertd527e in mice affects neither fertility nor the maternal transcriptome. While this evolving gene is not showing a notable function in laboratory mice, its documented evolutionary history in Muroidea during the last ~40 million years provides a textbook example of how a several common mutation events can support de novo gene formation, evolution of protein-coding capacity, as well as gene’s demise.
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| Overall design |
Comparative gene expression profiling analysis of RNA-seq data of GV stage oocytes from chinese hamster (Cricetulus griseus): - 2x wild-type
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| Contributor(s) |
Svoboda P, Malik R, Petrzilek J, Horvat F |
| Citation(s) |
36482406 |
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| Submission date |
Sep 20, 2022 |
| Last update date |
Jan 04, 2023 |
| Contact name |
Filip Horvat |
| E-mail(s) |
filip.horvat@img.cas.cz
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| Organization name |
Institute of Molecular Genetics of the ASCR
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| Lab |
Laboratory of Epigenetic Regulations
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| Street address |
Videnska 1083
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| City |
Prague |
| ZIP/Postal code |
14220 |
| Country |
Czech Republic |
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| Platforms (1) |
| GPL19001 |
Illumina HiSeq 2000 (Cricetulus griseus) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE213820 |
De novo emergence, existence, and demise of a protein-coding gene in murids |
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| Relations |
| BioProject |
PRJNA882483 |
