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| Status |
Public on Mar 06, 2023 |
| Title |
Bombyx Vasa sequesters transposon mRNAs in nuage via phase separation requiring RNA binding and self-association [RIP-Seq] |
| Organism |
Bombyx mori |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Bombyx Vasa (BmVasa) assembles non-membranous organelle, nuage or Vasa bodies, in germ cells, known as the center for Siwi-dependent transposon silencing and concomitant Ago3-piRISC biogenesis. However, details of the body assembly remain unclear. Here, we show that the N-terminal intrinsically disordered region (N-IDR) and RNA helicase domain of BmVasa are responsible for self-association and RNA binding, respectively, but N-IDR is also required for full RNA-binding activity. Both domains are essential for Vasa body assembly in vivo and droplet formation in vitro via phase separation. FAST-iCLIP reveals that BmVasa preferentially binds transposon mRNAs. Loss of Siwi function derepresses transposons but has marginal effects on BmVasa-RNA binding. This study shows that BmVasa assembles nuage by phase separation via its ability to self-associate and bind newly exported transposon mRNAs. This unique property of BmVasa allows transposon mRNAs to be sequestered and enriched in nuage, resulting in effective Siwi-dependent transposon repression and Ago3-piRISC biogenesis.
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| |
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| Overall design |
BmVasa binding RNAs (BmVasa FAST-iCLIP) in BmN4 cells
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| |
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| Contributor(s) |
Yamazaki H, Namba Y, Siomi MC |
| Citation(s) |
37029111 |
| |
| Submission date |
Sep 22, 2022 |
| Last update date |
May 11, 2023 |
| Contact name |
Yurika Namba |
| E-mail(s) |
yurika-namba@g.ecc.u-tokyo.ac.jp
|
| Organization name |
The University of Tokyo
|
| Department |
Department of Biological Sciences, Graduate School of Science
|
| Lab |
Siomi Lab
|
| Street address |
2-11-16 Yayoi, Bunkyo-ku
|
| City |
Tokyo |
| State/province |
--- Select a state --- |
| ZIP/Postal code |
113-0032 |
| Country |
Japan |
| |
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| Platforms (1) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE213917 |
Bombyx Vasa sequesters transposon mRNAs in nuage via phase separation requiring RNA binding and self-association |
|
| Relations |
| BioProject |
PRJNA883171 |
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