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| Status |
Public on Dec 04, 2023 |
| Title |
Bivalent histone modification of BCG1 enables fungal pathogen to evade host immunity (ChIP-Seq) |
| Organism |
Fusarium graminearum PH-1 |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Bivalent chromatin modification containing opposing H3K4me3 and H3K27me3 marks controls various biological processes by fine-tuning gene expression in animals and plants, however how this bivalent modification regulates pathogenicity of fungal pathogen remains exclusive. Here, we provided a genome-wide landscape of H3K4me3 and H3K27me3 of wheat head blight fungus Fusarium graminearum (Fg), leading to the identification of infection-induced bivalent chromatin-marked genes (BCGs). Among those, BCG1, which encodes a novel xylanase with a G/Q rich motif, is required for the full virulence of Fg pathogenicity through degradation of host cell wall. However, the G/Q rich motif is recognized by pattern-recognition receptors and triggers plant innate immunity. Further data illustrates that Fg employs H3K4me3 modification to induce BCG1 expression rapidly during the early infection, and then switches to bivalent H3K4me3-H3K27me3 chromatin state that renders rapid epigenetic silencing of BCG1 for escaping from host immune monitor, therefore leading to the successful invasion. Collectively, our study highlights the molecular mechanism of how fungal pathogen employs bivalent epigenetic modification to facilitate the successful infection by escaping of host immunity, which provides conceptual insights into plant-microbe interaction.
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| Overall design |
It was proved by ChIP-seq data and sequential ChIPd ata that modulation of BCG1 expression dynamics by bivalent histone methylations enables fungal pathogen to evade host immunity
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| Contributor(s) |
Gu Q, Zhao X, Yuan B |
| Citation missing |
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| |
| Submission date |
Sep 22, 2022 |
| Last update date |
Dec 05, 2023 |
| Contact name |
zhao xiao zhen |
| E-mail(s) |
zhaoxiaozhen@ahau.edu.cn
|
| Phone |
17768116791
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| Organization name |
Anhui Agricultural University
|
| Street address |
Anhui Agricultural University, 130 Changjiangxilu, Shushan District, Hefei, Anhui Province, China
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| City |
Hefei |
| ZIP/Postal code |
230036 |
| Country |
China |
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| Platforms (1) |
| GPL32260 |
Illumina HiSeq 4000 (Fusarium graminearum PH-1) |
|
| Samples (22)
|
| GSM6597001 |
ChIP-Seq of Fusarium graminearum PH-1:PH-1_Input rep1 |
| GSM6597002 |
ChIP-Seq of Fusarium graminearum PH-1:PH-1_Input rep2 |
| GSM6597003 |
ChIP-Seq of Fusarium graminearum PH-1:H3K4me3_IP rep1 |
| GSM6597004 |
ChIP-Seq of Fusarium graminearum PH-1:H3K4me3_IP rep2 |
| GSM6597005 |
ChIP-Seq of Fusarium graminearum PH-1:H3K27me3_IP rep1 |
| GSM6597006 |
ChIP-Seq of Fusarium graminearum PH-1:H3K27me3_IP rep2 |
| GSM6597007 |
ChIP-Seq of Fusarium graminearum PH-1:H3K4me3-H3K27me3_ip rep1 |
| GSM6597008 |
ChIP-Seq of Fusarium graminearum PH-1:H3K4me3-H3K27me3_ip rep2 |
| GSM6597009 |
ChIP-Seq of Fusarium graminearum PH-1:PH-1 infection_Input rep1 |
| GSM6597010 |
ChIP-Seq of Fusarium graminearum PH-1:PH-1 infection_Input rep2 |
| GSM6597011 |
ChIP-Seq of Fusarium graminearum PH-1:H3K4me3 in the PH-1 infection_ip rep1 |
| GSM6597012 |
ChIP-Seq of Fusarium graminearum PH-1:H3K4me3 in the PH-1 infection_ip rep2 |
| GSM6597013 |
ChIP-Seq of Fusarium graminearum PH-1:H3K27me3 in the PH-1 infection_ip rep1 |
| GSM6597014 |
ChIP-Seq of Fusarium graminearum PH-1:H3K27me3 in the PH-1 infection_ip rep2 |
| GSM6597015 |
ChIP-Seq of Fusarium graminearum PH-1: PH-1 infection-sequential ChIP_input rep1 |
| GSM6597016 |
ChIP-Seq of Fusarium graminearum PH-1:l PH-1 infection-sequential ChIP_input rep2 |
| GSM6597017 |
ChIP-Seq of Fusarium graminearum PH-1:H3K4me3-H3K27me3 in the PH-1 infection_ip rep1 |
| GSM6597018 |
ChIP-Seq of Fusarium graminearum PH-1:H3K4me3-H3K27me3 in the PH-1 infection_ip rep2 |
| GSM7300437 |
ChIP-Seq of Fusarium graminearum PH-1:H3K27me3-H3K4me3_ip_1 |
| GSM7300438 |
ChIP-Seq of Fusarium graminearum PH-1:H3K27me3-H3K4me3_ip_2 |
| GSM7300439 |
ChIP-Seq of Fusarium graminearum PH-1:H3K27me3-H3K4me3 in the PH-1 infection_ip_1 |
| GSM7300440 |
ChIP-Seq of Fusarium graminearum PH-1:H3K27me3-H3K4me3 in the PH-1 infection_ip_2 |
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| This SubSeries is part of SuperSeries: |
| GSE213962 |
Temporally-coordinated bivalent histone modifications of BCG1 enable fungal invasion and immune evasion |
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| Relations |
| BioProject |
PRJNA883379 |
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