NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE214029 Query DataSets for GSE214029
Status Public on Oct 11, 2023
Title UBE2A/B is the trans-acting factor mediating mechanotransduction and contact inhibition
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Mechanotransduction converts mechanical stimuli into biochemical signals to regulate cell growth, differentiation, and survival. However, how mechanotransduction is linked to gene expression remains poorly understood. Here, we report the identification of UBE2A as an mechano-sensitive trans-acting factor, which translocates from the cytosol to the nucleus upon mechanical stimuli. UBE2A is an E2 enzyme that catalyzes ubiquitination of histone, which eventually results in transcription activation of downstream genes. Using ChIP-seq and RNAseq analysis, we identify downstream genes of UBE2A, including YAP1, a well-known mechanotransducer. Knock-down of UBE2A/B significantly downregulates YAP1 protein expression. Our findings identify the new mechanotransduction pathway that regulates gene expression through histone ubiquitination by UBE2A/B.
 
Overall design Examination of trans-acting factors involved in mechanotransduction, identify candidates' biological function using RNA-seq in KD/normal HEK cell.
mRNA profiles of wild type (WT) and UBE2A/B knock-down (KD) HEK293 cells.
Web link https://portlandpress.com/biochemj/article/doi/10.1042/BCJ20230208/233610/UBE2A-B-is-the-trans-acting-factor-mediating
 
Contributor(s) Nakamura F, Feng M
Citation(s) 37818922
Submission date Sep 23, 2022
Last update date Oct 12, 2023
Contact name Fumihiko Nakamura
E-mail(s) fnakamura@tju.edu.cn
Organization name Tianjin University
Street address 92 Weijin Road, Nankai District
City Tianjin
ZIP/Postal code 300072
Country China
 
Platforms (1)
GPL20795 HiSeq X Ten (Homo sapiens)
Samples (6)
GSM6598089 UBE2AB_KD_Rep1
GSM6598090 UBE2AB_KD_Rep2
GSM6598091 UBE2AB_KD_Rep3
Relations
BioProject PRJNA883657

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE214029_Expression12.xlsx 12.5 Mb (ftp)(http) XLSX
GSE214029_Expression3.xlsx 5.2 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap
External link. Please review our privacy policy.