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| Status |
Public on Jan 12, 2023 |
| Title |
Gene expression profile at single embryo level of 0-3h old Drosophila embryos |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We used single-embryo sequencing to characterize early zygotic gene expression in Drosophila. The data provide the first continuous timeline of transcript levels during early development (≤ 3 hours) in Drosophila melanogaster.
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| Overall design |
RNA from single Drosophila embryos was isolated and subjected to a modified CelSeq2 single-cell RNA-sequencing protocol. Pseudo-temporal analysis of the single embryo transcriptomes provides a high-resolution, time-sensitive picture of transcriptional events during early embryonic development.
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| Contributor(s) |
Lempradl A, Perez-Mojica JE, Lau KH |
| Citation(s) |
36950383, 37682716 |
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| Submission date |
Sep 24, 2022 |
| Last update date |
Sep 20, 2023 |
| Contact name |
Adelheid Lempradl |
| E-mail(s) |
heidi.lempradl@vai.org
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| Phone |
6162345846
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| Organization name |
Van Andel Institute
|
| Department |
Metabolism and Nutritional Programing
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| Street address |
333 Bostwick Ave NE
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| City |
Grand Rapids |
| State/province |
Michigan |
| ZIP/Postal code |
49503 |
| Country |
USA |
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| Platforms (1) |
| GPL25244 |
Illumina NovaSeq 6000 (Drosophila melanogaster) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA883969 |
