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| Status |
Public on Oct 10, 2023 |
| Title |
35S:TRAP of S. lycopersicum and S. pennellii roots under phosphate-deprivation |
| Organisms |
Solanum lycopersicum; Solanum pennellii |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Phosphate is a necessary macronutrient for basic biological processes, plant growth, and agriculture. Plants modulate their root system architecture and cellular processes to adapt to phosphate deprivation albeit with a growth penalty. Excess application of phosphate in the form of fertilizer can lead to eutrophication and has negative environmental impact. Moreover, phosphate mined from rock reserves is a finite and non-recyclable resource and its levels are nearing complete depletion. Here, we show that Solanum pennellii, a wild relative of tomato, is partially insensitive to phosphate deprivation. Furthermore, it mounts a constitutive response under phosphate sufficiency. We demonstrate that activated brassinosteroid signaling through a tomato BZR1 ortholog gives rise to the same constitutive phosphate deficiency response which is dependent on over-accumulation of zinc. Collectively, these results reveal an additional strategy by which plants can adapt to phosphate starvation.
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| Overall design |
35S:TRAP construct-containing tomato seedlings were harvested between eight and ten days after germination. Two tissues were extracted for analysis, the root tip and the root midsection. A total of 4 biological replicates for each genotype and treatment were used with between 30 and 40 plants in each biological replicate.
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| Contributor(s) |
Gibson D, Demirer GS, Brady SM |
| Citation(s) |
37306070 |
| Submission date |
Sep 26, 2022 |
| Last update date |
Jan 10, 2024 |
| Contact name |
Siobhan Brady |
| E-mail(s) |
sbrady@ucdavis.edu
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| Organization name |
UC Davis
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| Department |
Plant Biology and Genome Center
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| Street address |
One Shields
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| City |
Davis |
| State/province |
California |
| ZIP/Postal code |
95616 |
| Country |
USA |
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| Platforms (2) |
| GPL16345 |
Illumina HiSeq 2000 (Solanum lycopersicum) |
| GPL22467 |
Illumina HiSeq 2000 (Solanum pennellii) |
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| Samples (32)
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| GSM6600859 |
Root tips, P sufficient, rep 4 [MST4] |
| GSM6600860 |
Root tips, P limiting, rep 1 [MLT1] |
| GSM6600861 |
Root tips, P limiting, rep 2 [MLT2] |
| GSM6600862 |
Root tips, P limiting, rep 3 [MLT3] |
| GSM6600863 |
Root tips, P limiting, rep 4 [MLT4] |
| GSM6600864 |
Root midsection, P sufficient, rep 1 [MSM1] |
| GSM6600865 |
Root midsection, P sufficient, rep 2 [MSM2] |
| GSM6600866 |
Root midsection, P sufficient, rep 3 [MSM3] |
| GSM6600867 |
Root midsection, P sufficient, rep 4 [MSM4] |
| GSM6600868 |
Root midsection, P limiting, rep 1 [MLM1] |
| GSM6600869 |
Root midsection, P limiting, rep 2 [MLM2] |
| GSM6600870 |
Root midsection, P limiting, rep 3 [MLM3] |
| GSM6600871 |
Root midsection, P limiting, rep 4 [MLM4] |
| GSM6600872 |
Root tips, P sufficient, rep 1 [PST1] |
| GSM6600873 |
Root tips, P sufficient, rep 2 [PST2] |
| GSM6600874 |
Root tips, P sufficient, rep 3 [PST3] |
| GSM6600875 |
Root tips, P sufficient, rep 4 [PST4] |
| GSM6600876 |
Root tips, P limiting, rep 1 [PLT1] |
| GSM6600877 |
Root tips, P limiting, rep 2 [PLT2] |
| GSM6600878 |
Root tips, P limiting, rep 3 [PLT3] |
| GSM6600879 |
Root tips, P limiting, rep 4 [PLT4] |
| GSM6600880 |
Root midsection, P sufficient, rep 1 [PSM1] |
| GSM6600881 |
Root midsection, P sufficient, rep 2 [PSM2] |
| GSM6600882 |
Root midsection, P sufficient, rep 3 [PSM3] |
| GSM6600883 |
Root midsection, P sufficient, rep 4 [PSM4] |
| GSM6600884 |
Root midsection, P limiting, rep 1 [PLM1] |
| GSM6600885 |
Root midsection, P limiting, rep 2 [PLM2] |
| GSM6600886 |
Root midsection, P limiting, rep 3 [PLM3] |
| GSM6600887 |
Root midsection, P limiting, rep 4 [PLM4] |
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| Relations |
| BioProject |
PRJNA884346 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE214195_M82_rm8_counts.csv.gz |
795.4 Kb |
(ftp)(http) |
CSV |
| GSE214195_Winter2017_35STRAP_Counts_Penn_MappedtoM82.csv.gz |
542.5 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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