|
| Status |
Public on Oct 15, 2010 |
| Title |
Transcriptional profiling of the T. thermophilus HB8 response to diamide |
| Organism |
Thermus thermophilus HB8 |
| Experiment type |
Expression profiling by array
|
| Summary |
We observed the expression profile of the total mRNA in Thermus thermophilus HB8 wild-type strain at 10 min after the addition of 10 mM diamide.
|
| |
|
| Overall design |
Three wild-type strain of T. thermophilus HB8 were pre-cultured at 70 degC for 16 hours in 4 mL of systhetic medium. The cells (2 mL) were inoculated into 250 mL of the same medium and then cultivated at 70°C for 8 hours (A600 value being ~0.34). Then the 10 mM of diamide was added into the medium, and continued cultivation. Cells were collected at 0 and 10 min time point after the addition of diamide, and then crude RNA was extracted. The expression of each mRNA at each time point was analyzed on a GeneChip.
|
| |
|
| Contributor(s) |
Agari Y, Ohmori M, Kuramitsu S, Shinkai A |
| Citation(s) |
21054499 |
| |
| Submission date |
Apr 21, 2010 |
| Last update date |
Mar 22, 2012 |
| Contact name |
Akeo Shinkai |
| E-mail(s) |
ashinkai@spring8.or.jp, y_agari@spring8.or.jp
|
| URL |
http://www.srg.harima.riken.jp/
|
| Organization name |
RIKEN Harima Institute
|
| Department |
SPring-8 Center
|
| Street address |
1-1-1, Kouto, Sayo
|
| City |
Hyogo |
| ZIP/Postal code |
679-5148 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL9209 |
[TTHB8401a520105F] Riken Thermus thermophilus HB8 4K mRNA custom array, library Rev.2 |
|
| Samples (6)
|
|
| This SubSeries is part of SuperSeries: |
| GSE21875 |
SuperSeries for the study of expression pattern analysis of Thermus thermophilus HB8 |
|
| Relations |
| BioProject |
PRJNA129485 |
Less...
More...