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Series GSE214433 Query DataSets for GSE214433
Status Public on Oct 16, 2023
Title Spatial-temporal tracking of Brca1-driven breast tumorigenesis from sporadic mutant cells with a genetic mosaic mouse model
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We utilized a genetic tool termed Mosaic Analysis with Double Markers (MADM) to achieve a sporadic loss of heterozygosity of Brca1 & Trp53 in mouse mammary epithelial cells and concomitantly label them with GFP. This MADM-based mouse model initiated cancer with sparse GFP+ mutant cells and developed mammary tumors that resemble human disease at pathological, transcriptomic, and genomic levels. This dataset provides bulk RNA sequencing of twelve mammary tumors from MADM Brca1-Trp53 animals.
 
Overall design Twelve MADM tumors from nine animals were homogenized and poly(A) RNA was sequenced.
 
Contributor(s) Janes KA, Zeng J, Singh S, Zong H
Citation(s) 37815460
Submission date Sep 29, 2022
Last update date Jan 17, 2024
Contact name Kevin A. Janes
E-mail(s) kjanes@virginia.edu
Organization name University of Virginia
Department Department of Biomedical Engineering
Street address Box 800759 Health System
City Charlottesville
State/province VA
ZIP/Postal code 22908
Country USA
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (12)
GSM6605763 MADM mammary tumor #1
GSM6605764 MADM mammary tumor #2
GSM6605765 MADM mammary tumor #3
Relations
BioProject PRJNA885400

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Supplementary file Size Download File type/resource
GSE214433_MADMrnaseq_TPM_geneid2022.csv.gz 1.6 Mb (ftp)(http) CSV
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