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Status |
Public on Oct 16, 2023 |
Title |
Spatial-temporal tracking of Brca1-driven breast tumorigenesis from sporadic mutant cells with a genetic mosaic mouse model |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We utilized a genetic tool termed Mosaic Analysis with Double Markers (MADM) to achieve a sporadic loss of heterozygosity of Brca1 & Trp53 in mouse mammary epithelial cells and concomitantly label them with GFP. This MADM-based mouse model initiated cancer with sparse GFP+ mutant cells and developed mammary tumors that resemble human disease at pathological, transcriptomic, and genomic levels. This dataset provides bulk RNA sequencing of twelve mammary tumors from MADM Brca1-Trp53 animals.
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Overall design |
Twelve MADM tumors from nine animals were homogenized and poly(A) RNA was sequenced.
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Contributor(s) |
Janes KA, Zeng J, Singh S, Zong H |
Citation(s) |
37815460 |
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Submission date |
Sep 29, 2022 |
Last update date |
Jan 17, 2024 |
Contact name |
Kevin A. Janes |
E-mail(s) |
kjanes@virginia.edu
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Organization name |
University of Virginia
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Department |
Department of Biomedical Engineering
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Street address |
Box 800759 Health System
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City |
Charlottesville |
State/province |
VA |
ZIP/Postal code |
22908 |
Country |
USA |
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Platforms (1) |
GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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Samples (12)
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Relations |
BioProject |
PRJNA885400 |