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Status |
Public on Oct 09, 2023 |
Title |
Comparison of bulk RNAseq results of WT and TKO E14 EpiLCs |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We investigate the impact of DNA methylation on epiblast differentiation. To this end, we used in vitro Epiblast-like cell (EpiLC) differentiation on WT and DNA-methylation free TKO murine ESCs. We next performed bulk RNAseq at D0, D2, D4, D6 and D8 of differentiation to study transcriptomic differences.
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Overall design |
Bulk mRNA sequencing on WT and TKO ESCs (D0), and D2, D4 and D7 cells during EpiLC differentiation
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Contributor(s) |
Schulz M, Teissandier A, Bourc'his D |
Citation missing |
Has this study been published? Please login to update or notify GEO. |
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Submission date |
Sep 29, 2022 |
Last update date |
Oct 10, 2023 |
Contact name |
teissandier aurelie |
E-mail(s) |
aurelie.teissandier@curie.fr
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Organization name |
institut curie
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Department |
u934
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Lab |
Epigenetic decisions and reproduction in mammals
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Street address |
26 rue d'Ulm
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City |
paris |
ZIP/Postal code |
75248 |
Country |
France |
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Platforms (1) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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Samples (16)
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GSM6606115 |
E14 WT cells, D0, biological replicate 1 (BulkRNAseq_EpiLC) |
GSM6606116 |
E14 WT cells, D0, biological replicate 2 (BulkRNAseq_EpiLC) |
GSM6606117 |
E14 WT cells, D2, biological replicate 1 (BulkRNAseq_EpiLC) |
GSM6606118 |
E14 WT cells, D2, biological replicate 2 (BulkRNAseq_EpiLC) |
GSM6606119 |
E14 WT cells, D4, biological replicate 1 (BulkRNAseq_EpiLC) |
GSM6606120 |
E14 WT cells, D4, biological replicate 2 (BulkRNAseq_EpiLC) |
GSM6606121 |
E14 WT cells, D7, biological replicate 1 (BulkRNAseq_EpiLC) |
GSM6606122 |
E14 WT cells, D7, biological replicate 2 (BulkRNAseq_EpiLC) |
GSM6606123 |
E14 TKO cells, D0, biological replicate 1 (BulkRNAseq_EpiLC) |
GSM6606124 |
E14 TKO cells, D0, biological replicate 2 (BulkRNAseq_EpiLC) |
GSM6606125 |
E14 TKO cells, D2, biological replicate 1 (BulkRNAseq_EpiLC) |
GSM6606126 |
E14 TKO cells, D2, biological replicate 2 (BulkRNAseq_EpiLC) |
GSM6606127 |
E14 TKO cells, D4, biological replicate 1 (BulkRNAseq_EpiLC) |
GSM6606128 |
E14 TKO cells, D4, biological replicate 2 (BulkRNAseq_EpiLC) |
GSM6606129 |
E14 TKO cells, D7, biological replicate 1 (BulkRNAseq_EpiLC) |
GSM6606130 |
E14 TKO cells, D7, biological replicate 2 (BulkRNAseq_EpiLC) |
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This SubSeries is part of SuperSeries: |
GSE214496 |
DNA methylation restricts default deterministic fates towards neural and germline lineages |
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Relations |
BioProject |
PRJNA885461 |
Supplementary file |
Size |
Download |
File type/resource |
GSE214459_RAW.tar |
27.5 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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