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Status |
Public on Oct 09, 2023 |
Title |
Comparison of bulk RNAseq results of WT and TKO E14 NPCs |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We investigate the impact of DNA methylation on neural somatic differentiation. To this end, we used in vitro neural progenitor cell (NPC) differentiation on WT and DNA-methylation free TKO murine ESCs. We next performed bulk RNAseq at D0, D2, D4, D6 and D8 of differentiation to study transcriptomic differences.
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Overall design |
Bulk mRNA sequencing on WT and TKO ESCs (D0), and D2, D4, D6 and D8 cells during NPC differentiation
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Contributor(s) |
Schulz M, De la mata santaella E, Teissandier A, Bourc'his D |
Citation missing |
Has this study been published? Please login to update or notify GEO. |
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Submission date |
Sep 29, 2022 |
Last update date |
Oct 10, 2023 |
Contact name |
teissandier aurelie |
E-mail(s) |
aurelie.teissandier@curie.fr
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Organization name |
institut curie
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Department |
u934
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Lab |
Epigenetic decisions and reproduction in mammals
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Street address |
26 rue d'Ulm
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City |
paris |
ZIP/Postal code |
75248 |
Country |
France |
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Platforms (1) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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Samples (20)
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GSM6606131 |
E14 WT cells, D0, biological replicate 1 (BulkRNAseq_NPC) |
GSM6606132 |
E14 WT cells, D0, biological replicate 2 (BulkRNAseq_NPC) |
GSM6606133 |
E14 WT cells, D2, biological replicate 1 (BulkRNAseq_NPC) |
GSM6606134 |
E14 WT cells, D2, biological replicate 2 (BulkRNAseq_NPC) |
GSM6606135 |
E14 WT cells, D4, biological replicate 1 (BulkRNAseq_NPC) |
GSM6606136 |
E14 WT cells, D4, biological replicate 2 (BulkRNAseq_NPC) |
GSM6606137 |
E14 WT cells, D6, biological replicate 1 (BulkRNAseq_NPC) |
GSM6606138 |
E14 WT cells, D6, biological replicate 2 (BulkRNAseq_NPC) |
GSM6606139 |
E14 WT cells, D8, biological replicate 1 (BulkRNAseq_NPC) |
GSM6606140 |
E14 WT cells, D8, biological replicate 2 (BulkRNAseq_NPC) |
GSM6606141 |
E14 TKO cells, D0, biological replicate 1 (BulkRNAseq_NPC) |
GSM6606142 |
E14 TKO cells, D0, biological replicate 2 (BulkRNAseq_NPC) |
GSM6606143 |
E14 TKO cells, D2, biological replicate 1 (BulkRNAseq_NPC) |
GSM6606144 |
E14 TKO cells, D2, biological replicate 2 (BulkRNAseq_NPC) |
GSM6606145 |
E14 TKO cells, D4, biological replicate 1 (BulkRNAseq_NPC) |
GSM6606146 |
E14 TKO cells, D4, biological replicate 2 (BulkRNAseq_NPC) |
GSM6606147 |
E14 TKO cells, D6, biological replicate 1 (BulkRNAseq_NPC) |
GSM6606148 |
E14 TKO cells, D6, biological replicate 2 (BulkRNAseq_NPC) |
GSM6606149 |
E14 TKO cells, D8, biological replicate 1 (BulkRNAseq_NPC) |
GSM6606150 |
E14 TKO cells, D8, biological replicate 2 (BulkRNAseq_NPC) |
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This SubSeries is part of SuperSeries: |
GSE214496 |
DNA methylation restricts default deterministic fates towards neural and germline lineages |
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Relations |
BioProject |
PRJNA885460 |
Supplementary file |
Size |
Download |
File type/resource |
GSE214460_RAW.tar |
34.4 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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