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| Status |
Public on Oct 06, 2023 |
| Title |
Effect of silencing of p53 on gene expression in MDA-MB 231 TNBC cell line in medium with 100% and medium with 25% of amino acids. [aa] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Mutant p53 can acquires oncogenic properties supporting tumor growth, metastases and chemoresistance, by reprogramming cancer cell transcriptome, proteome and metabolome. To investigate what is the gene expression network regulated by mutant p53 in condition of limited amino acids availability, we silenced mutant p53 expression in MDA-MB-231 Triple Negative Breast Cancer (TNBC) cell line, grown in medium with 100% and medium with 25% of amino acids.
We performed gene expression profiling analysis using data obtained from RNA-seq of MDA-MB 231.
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| Overall design |
Comparative gene expression profiling analysis of RNA-seq data for MDA-MB 231 cells, grown in medium with 100% and medium with 25% of amino acids and transfected with Control or p53 siRNAs, each in duplicate.
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| Contributor(s) |
Tombari C, Zannini A, Bicciato S, Del Sal G |
| Citation(s) |
37880212 |
| |
| Submission date |
Sep 29, 2022 |
| Last update date |
Nov 13, 2023 |
| Contact name |
Silvio Bicciato |
| E-mail(s) |
silvio.bicciato@unipd.it
|
| Phone |
+39-049-827-6108
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| Organization name |
University of Padova
|
| Department |
Molecular Medicine
|
| Street address |
via U. Bassi 59/b
|
| City |
Padova |
| ZIP/Postal code |
35131 |
| Country |
Italy |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE214494 |
Mutant p53 regulates serine-glycine synthesis and essential amino acids intake |
|
| Relations |
| BioProject |
PRJNA885517 |
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