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Status |
Public on Oct 15, 2010 |
Title |
Expression profile of TTHA1939-deficient Thermus thermophilus HB8 strain |
Organism |
Thermus thermophilus HB8 |
Experiment type |
Expression profiling by array
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Summary |
We observed the expression profile of the total mRNA of the TTHA1939-deficient strain grown in a rich medium at 70°C for 800 min.
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Overall design |
Wild-type and the mutant strains were precultured in rich medium (TR medium) for two times at 70 oC and then subcultured to minimum essential medium (CS medium). These cells were harvested during the logarithmic growth phase (800 minutes from the start of culture). Total RNA were extracted from each strain and used for the cDNA synthesis. For the biological replication, above experiments were performed four times independently.
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Contributor(s) |
Agari Y, Ohmori M, Ooga T, Kuramitsu S, Shinkai A |
Citation(s) |
21054499 |
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Submission date |
Apr 22, 2010 |
Last update date |
Mar 22, 2012 |
Contact name |
Akeo Shinkai |
E-mail(s) |
ashinkai@spring8.or.jp, y_agari@spring8.or.jp
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URL |
http://www.srg.harima.riken.jp/
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Organization name |
RIKEN Harima Institute
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Department |
SPring-8 Center
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Street address |
1-1-1, Kouto, Sayo
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City |
Hyogo |
ZIP/Postal code |
679-5148 |
Country |
Japan |
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Platforms (1) |
GPL9209 |
[TTHB8401a520105F] Riken Thermus thermophilus HB8 4K mRNA custom array, library Rev.2 |
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Samples (4)
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This SubSeries is part of SuperSeries: |
GSE21875 |
SuperSeries for the study of expression pattern analysis of Thermus thermophilus HB8 |
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Relations |
BioProject |
PRJNA129537 |